LOCUS       sequence-99169-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty backbone for fusing gene of interest to N-term of mKO.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mKusabira Orange-N1
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(126..782)
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
     regulatory      779..788
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc tagagtcgcg
      121 gccgcttagg aatgagctac tgcatcttct accagctcag taatgttgcc ttcggttttc
      181 ctgacgaggc gatggctgat gtaatggctt cctggcattt taagaatctt ttttgccgcc
      241 ttgtaagtag tcttgaattg gcatttgtga ttgccgcctc cttcaagttt taggtacatc
      301 gtaacatcac ccttcagaac tccgtcgctg gcagtaattt tctcggttga tggctcccaa
      361 tcaacacttt ggttttgcat gataggacca tcggcaggaa agttaacccc agtaaatttg
      421 gatttgtggt agaaggtgtt tcctctaagg cttatatgcg cactgactga agcggaccca
      481 ccatcttcga actccaacga cctttcccat gacaggcctt caggaaatgc ttgtttgaaa
      541 tagtctggta tctcttctgg atatttagta aaaggtctgt ggccgtaaca gaacacgtgt
      601 gacactaagt caaacgcgaa aggcattggc ccgcccttgg ccattgtgac gcgtagtgtc
      661 atctcttgat gtccctcgta aggtctgcct gtgccttcac cttcaattgt gaactcatgc
      721 ccattgacgg agccgtccat gtagtacctc atcttcatct ctggtttaat cacactcacc
      781 atggtggcga ccggtggatc ccgggcccgc ggtaccgtcg actgcagaat tcgatgcctc
      841 agaactttgg tgtcattggt ctcagacacc actttgccat ccactatccg gcgg
//
