LOCUS       sequence-99170-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses PAD (phenolic acid decarboxylase).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Tomek KJ, Saldarriaga CR, Velasquez FP, Liu T, Hodge DB, Whitehead 
            TA
  TITLE     Removal and upgrading of lignocellulosic fermentation inhibitors by 
            in situ biocatalysis and liquid-liquid extraction.
  JOURNAL   Biotechnol Bioeng. 2015 Mar;112(3):627-32. doi: 10.1002/bit.25473. 
            Epub 2014 Nov 24.
  PUBMED    25311910
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.25473";
            journalName: "Biotechnol Bioeng"; date: "2015-03"; volume: "112"; 
            issue: "3"; pages: "627-32"
COMMENT     Sequence Label: pJK_kanR_proJK1_PAD
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..28)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             531..553
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ccggatctca gtggtggtgg tggtggtgct cgagtaagcg acctgctcta atgtcatctg
       61 tcatgccttc gtatggggcg taggaaatca cttcttcgtt gtcaacgcct tcattcttca
      121 ggaaagtgat ttctgcaaat tccggcacaa catattttgg gtaggtttca tacttttcac
      181 gagattcttt catcaagtcg atgtgatcgt tctggtaaca cacggtaatt tctgggtgtt
      241 catgaaccca cttagggaaa aagataatac catgcatgcg tttttcgtta ggcatgaagt
      301 tcaatgagac atcagtgccg gtcggttccg tccaagacac cttgtaaaca ccttctgtca
      361 gctttacgat gttgacttct tggtcacgaa cccaacggcc agcgaccata ccgctatgga
      421 tacggtaatc aatggtgtgg tcgtttttga tgtagatttc gtattcccaa ccgttttcgt
      481 aagtgtagat catgtgtgag ccgataaagt tttccatgcc catatgtata tctccttctt
      541 aaagttaaac aaaattattt ctagaaagtt aaacaaaatt atttgtagag ggaaaccgtt
      601 gtggtctccc tgaatatatt atacgagcct tatgcatgcc cataaagtta tccagcaacc
      661 actcatagac ctagggcagc agatagggac gacgtggtgt tagctgtggc cggcatggcg
      721 gccccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt
      781 tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc
      841 tgcaaaacgt ctgcgacctg agcaacaaca tgaa
//
