LOCUS       sequence-99173-e         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression of v-H-Ras and luciferase/EGFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Holczbauer A, Factor VM, Andersen JB, Marquardt JU, Kleiner DE, 
            Raggi C, Kitade M, Seo D, Akita H, Durkin ME, Thorgeirsson SS
  TITLE     Modeling pathogenesis of primary liver cancer in lineage-specific 
            mouse cell types.
  JOURNAL   Gastroenterology. 2013 Jul;145(1):221-31. doi: 
            10.1053/j.gastro.2013.03.013. Epub 2013 Mar 19.
  PUBMED    23523670
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1053/j.gastro.2013.03.013"; journalName: "Gastroenterology"; 
            date: "2013-07"; volume: "145"; issue: "1"; pages: "221-31"
COMMENT     Sequence Label: pSico-CAG-v-H-Ras-IRES-Luciferase/EGFP
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    49..73
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(644..668)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccttcttctc tttcctctcc tgacaggttg gtgtacagta gcttccaccc aactttgtac
       61 aaaaaagttg gcaccatgac agaatacaag cttgtggtgg tgggcgctag aggcgtggga
      121 aagagtgccc tgaccatcca gctgatccag aaccattttg tggacgagta tgatcccact
      181 atagaggact cctaccggaa acaggtagtc attgatgggg agacgtgttt actggacatc
      241 ttagacacaa caggtcaaga agagtatagt gccatgcggg accagtacat gcgcacaggg
      301 gagggcttcc tctgtgtatt tgccatcaac aacaccaagt cctttgaaga catccatcag
      361 tacagggagc agatcaagcg ggtgaaagat tcagatgatg tgccaatggt gctggtgggc
      421 aacaagtgtg acctggccgc tcgcactgtt gagtctcggc aggcccagga ccttgctcgc
      481 agctatggca tcccctacat tgaaacatca gccaagaccc ggcagggtgt agaggatgcc
      541 ttctacacac tagtacgtga gattcggcag cataaactgc ggaaactgaa cccgcctgat
      601 gagagtggcc ctggctgcat gagctgcaag tgtgtgctgt cctaatcaac tttcttgtac
      661 aaagttgtga tcaagggatc caatctcgag gcccctctcc ctcccccccc cctaacgtta
      721 ctggccgaag cc
//
