LOCUS       sequence-99174-e        1170 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant protein expression of Isocyanide Hydratase from 
            Pseudomonas fluorescens.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Lakshminarasimhan M, Madzelan P, Nan R, Milkovic NM, Wilson MA
  TITLE     Evolution of new enzymatic function by structural modulation of 
            cysteine reactivity in Pseudomonas fluorescens isocyanide hydratase.
  JOURNAL   J Biol Chem. 2010 Sep 17;285(38):29651-61. doi: 
            10.1074/jbc.M110.147934. Epub 2010 Jul 14.
  PUBMED    20630867
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M110.147934"; journalName: "J Biol Chem"; date: 
            "2010-09-17- 17"; volume: "285"; issue: "38"; pages: "29651-61"
COMMENT     Sequence Label: PfICH pET15b
FEATURES             Location/Qualifiers
     source          1..1170
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             91..108
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttgagaaatt ccctctgaat aattttgttt actttaagaa ggagatatac catgggcagc
       61 agccatcatc attatcatca cagcagcggc ctggtgccgc gcggcagcca tatggcagtg
      121 cagataggtt ttttgttgtt ccccgaggtc cagcaactgg atctgaccgg tccccacgac
      181 gtactggcct cgctgccgga tgtgcaggtg cacctgatct ggaaggagcc ggggccggtg
      241 gtggccagct cggggctggt gctgcaggcc accacaagct tcgccgactg cccgccgctg
      301 gatgtgatct gcatccccgg cggtaccggg gtcggggcgc tgatggaaga cccgcaggcc
      361 ctggcgttca tccgccagca ggccgcccgg gcgcgctacg tgacctcggt gtgcaccggc
      421 tccctggtgc tcggcgccgc gggcctgctc cagggcaagc gcgccaccac ccactgggcc
      481 taccacgaac tgctggcgcc actgggggcg ataccggtgc atgagcgggt ggtgcgtgac
      541 ggcaacctgc tgaccggcgg cggtatcacc gccggcatcg acttcgccct gaccctggcc
      601 gccgaactgt tcgacgccgc caccgcgcag cgggtacagc tgcaactgga gtacgccccg
      661 gcgccgccgt tcaatgccgg cagcccggac acggcccccg ccagcgtggt gcagcaggcc
      721 cgccagcgcg ccgcggattc gctgcacaag cgcagggaaa tcaccctgcg cgcggccgcg
      781 cggctggcag ccggctgact cgaggatccg gctgctaaca aagcccgaaa ggaagctgag
      841 ttggctgctg ccaccgctga ncaataacta gcataacccc ttgggggcct tctaaacggg
      901 tcttgagggg ttttttgctg aaaggaggaa ctatatccng gatatcccgc aagaagcccg
      961 ggcagtancg gcataaccaa gccctatgcc tanancatcc aagggtgaac ggtgcccaag
     1021 aataacnatn aacgcattnt tnaaattttc atacacggtg cctgaantgc gttaaacnat
     1081 tttaanctgt gantaaaata ncncaattaa aantttatnn atgaanaaac tnttnaaaaa
     1141 ttgaaaattn ttttaaaaan aaaaaggcct
//
