LOCUS       sequence-99175-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant protein expression of Isocyanide Hydratase from 
            Pseudomonas fluorescens.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Lakshminarasimhan M, Madzelan P, Nan R, Milkovic NM, Wilson MA
  TITLE     Evolution of new enzymatic function by structural modulation of 
            cysteine reactivity in Pseudomonas fluorescens isocyanide hydratase.
  JOURNAL   J Biol Chem. 2010 Sep 17;285(38):29651-61. doi: 
            10.1074/jbc.M110.147934. Epub 2010 Jul 14.
  PUBMED    20630867
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M110.147934"; journalName: "J Biol Chem"; date: 
            "2010-09-17- 17"; volume: "285"; issue: "38"; pages: "29651-61"
COMMENT     Sequence Label: PfICH pET15b
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(709..726)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(736..753)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agcagccgga tcctcgagtc agccggctgc cagccgcgcg gccgcgcgca gggtgatttc
       61 cctgcgcttg tgcagcgaat ccgcggcgcg ctggcgggcc tgctgcacca cgctggcggg
      121 ggccgtgtcc gggctgccgg cattgaacgg cggcgccggg gcgtactcca gttgcagctg
      181 tacccgctgc gcggtggcgg cgtcgaacag ttcggcggcc agggtcaggg cgaagtcgat
      241 gccggcggtg ataccgccgc cggtcagcag gttgccgtca cgcaccaccc gctcatgcac
      301 cggtatcgcc cccagtggcg ccagcagttc gtggtaggcc cagtgggtgg tggcgcgctt
      361 gccctggagc aggcccgcgg cgccgagcac cagggagccg gtgcacaccg aggtcacgta
      421 gcgcgcccgg gcggcctgct ggcggatgaa cgccagggcc tgcgggtctt ccatcagcgc
      481 cccgaccccg gtaccgccgg ggatgcagat cacatccagc ggcgggcagt cggcgaagct
      541 tgtggtggcc tgcagcacca gccccgagct ggccaccacc ggccccggct ccttccagat
      601 caggtgcacc tgcacatccg gcagcgaggc cagtacgtcg tggggaccgg tcagatccag
      661 ttgctggacc tcggggaaca acaaaaaacc tatctgcact gccatatggc tgccgcgcgg
      721 caccaggccg ctgctgtgat gatgatgatg atggctgctg cccatggtat atctccttct
      781 taaagttaaa c
//
