LOCUS       sequence-99177-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express VWF A1-GPIba ReaLiSM construct in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Kim J, Zhang CZ, Zhang X, Springer TA
  TITLE     A mechanically stabilized receptor-ligand flex-bond important in the
            vasculature.
  JOURNAL   Nature. 2010 Aug 19;466(7309):992-5. doi: 10.1038/nature09295.
  PUBMED    20725043
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-08-19- 19"; volume: "466"; issue: "7309"; 
            pages: "992-5"
COMMENT     Sequence Label: ReaLiSM A1-26link-GPIba+2cys
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..49)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     regulatory      751..760
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(825..844)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 tctcaccagt ggtgctggag ctggatccgc cttgaaaata aagattctcc ccggtaccag
       61 gaggggcttc aggggcaagg tcacagaggt agctaacgat ctcgtccctt tgctgctcca
      121 gctcatccac actgctcagc acgaaggcct tgttctcagg ggcctgcttc tcgatgaggc
      181 ggatctgctt gaggttggca tggggcccaa tgcccaccgg gatcacaatg accttcttct
      241 tcttcaggcc ctggacgtag cggacaaagt tccgggacat ccgttggggc tcctggctgg
      301 ccatcaggag cagggcgatg cgggaggctt cagggcggtc gatcttgctg aagatttgga
      361 acagtgtgta tttcaagacc tcgctggtgg aggccacctg gctgcccgca tacttcacct
      421 ggctggcaat gcgccgcagc tctgatggtc gcttccggtc cttgagcccg atgtaggcgt
      481 gggagccgtc gtggtactcc accacggcca cgcggaccca cttctgggag atgcgcagcc
      541 gctccatcat gtccaccaca aaggccttca gcacttcaaa ctcagcctcg gacagcctgg
      601 aggagccatc cagcaggaag accaggtcca gtagcctgct gcagtagaaa tcgtgcaacg
      661 gcggttccga gatgtctccg gagcacttcc ccaacacccc actgaaccag agcaagagca
      721 accccagcaa ttgagcagga actctcatat ccatggtggc gtacgtcgac gctagcgaat
      781 tcggcctgtc acggccgcaa ttctttgcca aaatgatgag acagcacaat aaccagcacg
      841 ttgcccagga gctgtaggaa
//
