LOCUS       sequence-99178-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant protein expression of Park7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Milkovic NM, Catazaro J, Lin J, Halouska S, Kizziah JL, Basiaga S, 
            Cerny RL, Powers R, Wilson MA
  TITLE     Transient sampling of aggregation-prone conformations causes 
            pathogenic instability of a parkinsonian mutant of DJ-1 at 
            physiological temperature.
  JOURNAL   Protein Sci. 2015 Oct;24(10):1671-85. doi: 10.1002/pro.2762. Epub 
            2015 Aug 17.
  PUBMED    26234586
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2762"; 
            journalName: "Protein Sci"; date: "2015-10"; volume: "24"; issue: 
            "10"; pages: "1671-85"
COMMENT     Sequence Label: DJ-1 TEV site pET15b
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(589..609)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(616..633)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(643..660)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             679..701
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    716..740
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(740..759)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(741..759)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(844..863)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gttagcagcc ggatcctcga gctagtcttt aagaacaagt ggagccttca cttgagccgc
       61 cacctccttg ccattcaggg cttcaacaat tgcaagcgca aactcgaagc tggtcccagg
      121 cccccggctt gtaagaatca ggccgtcttt ttccacacga ttctcagagt aggtgtaatg
      181 acctccattc atcattttgt ctttagcaag agggtgtgtt gtaactttac ttccaaaacc
      241 tatttcatga gccaacagag cagtaggacc tgcacagatg gcggctatca ggcccttccg
      301 gttttcctgc tccttcagta tctccttcac agcagcagac tcagataaat tctgtgcgcc
      361 cagattacct cctggtagaa ccaccacatc ataaggtccc tctttttttg catcttcaag
      421 gctggcatca ggacaaatga ccacatcacg gctacactgt actgggtctt ttccagccag
      481 gcctgcaacg gtgaccttaa tcccagctcg cctcatgaca tctacaggga tgaccgtctc
      541 catttcctct gctcctttag ccaggatgac cagagctctt ttggaagcag actgaaaata
      601 caggttttcc atatggctgc cgcgcggcac caggccgctg ctgtgatgat gatgatgatg
      661 gctgctgccc atggtatatc tccttcttaa agttaaacaa aattatttct agaggggaat
      721 tgttatccgc tcacaattcc cctatagtga gtcgtattaa tttcgcggga tcgagatctc
      781 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
      841 cgccctatat cgccgacatc accgat
//
