LOCUS       sequence-99179-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express VWF A1-GPIba ReaLiSM construct in mammalian cells  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Kim J, Zhang CZ, Zhang X, Springer TA
  TITLE     A mechanically stabilized receptor-ligand flex-bond important in the
            vasculature.
  JOURNAL   Nature. 2010 Aug 19;466(7309):992-5. doi: 10.1038/nature09295.
  PUBMED    20725043
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-08-19- 19"; volume: "466"; issue: "7309"; 
            pages: "992-5"
COMMENT     Sequence Label: ReaLiSM A1-Hinge-GPIba+2cys
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      759..768
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tgctggtttt ggaggcttag gcggcttgat tgtgggcccg gtaccagtag gaggaggggc
       61 ttcaggggca aggtcacaga ggtagctaac gatctcgtcc ctttgctgct ccagctcatc
      121 cacactgctc agcacgaagg ccttgttctc aggggcctgc ttctcgatga ggcggatctg
      181 cttgaggttg gcatggggcc caatgcccac cgggatcaca atgaccttct tcttcttcag
      241 gccctggacg tagcggacaa agttccggga catccgttgg ggctcctggc tggccatcag
      301 gagcagggcg atgcgggagg cttcagggcg gtcgatcttg ctgaagattt ggaacagtgt
      361 gtatttcaag acctcgctgg tggaggccac ctggctgccc gcatacttca cctggctggc
      421 aatgcgccgc agctctgatg gtcgcttccg gtccttgagc ccgatgtagg cgtgggagcc
      481 gtcgtggtac tccaccacgg ccacgcggac ccacttctgg gagatgcgca gccgctccat
      541 catgtccacc acaaaggcct tcagcacttc aaactcagcc tcggacagcc tggaggagcc
      601 atccagcagg aagaccaggt ccagtagcct gctgcagtag aaatcgtgca acggcggttc
      661 cgagatacaa ccggtttcag ctacgcaacc catcagcagc acggagagaa ggctcacgag
      721 ggagagcagc gcaggcatcc cagggctggg aaggatcccc atggtggcaa gcttgaattc
      781 ggcctgagac ggccgcaaat tctttgccaa aatgatgaga cagcacaata acca
//
