LOCUS       sequence-99180-e         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant protein expression of DJ-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Madzelan P, Labunska T, Wilson MA
  TITLE     Influence of peptide dipoles and hydrogen bonds on reactive cysteine
            pKa values in fission yeast DJ-1.
  JOURNAL   FEBS J. 2012 Nov;279(22):4111-20. doi: 10.1111/febs.12004. Epub 2012
            Oct 11.
  PUBMED    22971103
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/febs.12004"; journalName: "FEBS J"; date: "2012-11"; 
            volume: "279"; issue: "22"; pages: "4111-20"
COMMENT     Sequence Label: SpDJ-1 pET15b
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(722..740)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atggttaaag tgtgcctgtt tgttgcagat
      121 ggcaccgatg aaattgaatt tagcgcaccg tggggtattt ttaaacgtgc cgaaattccg
      181 attgatagcg tgtatgtggg cgaaaataaa gatcgtctgg tgaaaatgag ccgtgatgtt
      241 gaaatgtatg ccaatcgcag ctataaagaa attccgagcg cagatgattt tgccaaacag
      301 tatgatattg ccattattcc gggtggtggt ctgggtgcaa aaaccctgag caccaccccg
      361 tttgttcagc aggttgtgaa agaattttat aaaaaaccga ataaatggat tggtatgatt
      421 tgtgcaggca ccctgaccgc aaaaaccagc ggtctgccga ataaacaaat taccggtcat
      481 ccgagcgttc gtggtcagct ggaagaaggt ggctataaat atctggatca gccggttgtt
      541 ctggaagaaa atctgattac cagccagggt ccgggtacag caatgctgtt tggtctgaaa
      601 ctgctggaac aggtggcaag caaagataaa tataatgccg tgtataaaag cctgagcatg
      661 ccgtaactcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
      721 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt ga
//
