LOCUS       sequence-99182-e         656 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant protein expression of DJ-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Madzelan P, Labunska T, Wilson MA
  TITLE     Influence of peptide dipoles and hydrogen bonds on reactive cysteine
            pKa values in fission yeast DJ-1.
  JOURNAL   FEBS J. 2012 Nov;279(22):4111-20. doi: 10.1111/febs.12004. Epub 2012
            Oct 11.
  PUBMED    22971103
REFERENCE   2  (bases 1 to 656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/febs.12004"; journalName: "FEBS J"; date: "2012-11"; 
            volume: "279"; issue: "22"; pages: "4111-20"
COMMENT     Sequence Label: SpDJ-1 pET15b
FEATURES             Location/Qualifiers
     source          1..656
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(598..615)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(625..642)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agcagccgga tcctcgagtt acggcatgct caggctttta tacacggcat tatatttatc
       61 tttgcttgcc acctgttcca gcagtttcag accaaacagc attgctgtac ccggaccctg
      121 gctggtaatc agattttctt ccagaacaac cggctgatcc agatatttat agccaccttc
      181 ttccagctga ccacgaacgc tcggatgacc ggtaatttgt ttattcggca gaccgctggt
      241 ttttgcggtc agggtgcctg cacaaatcat accaatccat ttattcggtt ttttataaaa
      301 ttctttcaca acctgctgaa caaacggggt ggtgctcagg gtttttgcac ccagaccacc
      361 acccggaata atggcaatat catactgttt ggcaaaatca tctgcgctcg gaatttcttt
      421 atagctgcga ttggcataca tttcaacatc acggctcatt ttcaccagac gatctttatt
      481 ttcgcccaca tacacgctat caatcggaat ttcggcacgt ttaaaaatac cccacggtgc
      541 gctaaattca atttcatcgg tgccatctgc aacaaacagg cacactttaa ccatatggct
      601 gccgcgcggc accaggccgc tgctgtgatg atgatgatga tggctgctgc ccatgg
//
