LOCUS       sequence-99185-e        1106 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MESA target chain with CD4 ectodomain, no linker domain, G cleavage 
            sequence, and tTA-BFP fusion .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Daringer NM, Dudek RM, Schwarz KA, Leonard JN
  TITLE     Modular Extracellular Sensor Architecture for Engineering Mammalian 
            Cell-based Devices.
  JOURNAL   ACS Synth Biol. 2014 Mar 11.
  PUBMED    24611683
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Mar 11."
COMMENT     Sequence Label: CD4_LD 0_CS G_tTA-BFP
FEATURES             Location/Qualifiers
     source          1..1106
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     832..853
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cantaagttg atagggacaa ggacctttaa tagaaattgg acagcaagaa agcgagctta
       61 gtgatacttg ttgggccagg gcattagcca caccagccac cactttctga taggcagcct
      121 gcactggtgg ggtgaattgc tcgaggttta aacagctcca ccgcggtggc ggccgctatt
      181 acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtgcggaac tccagcagga
      241 ccatgtgatc gcgcttctcg ttggggtctt tgctcagcac ggactgggtg ctcaggtagt
      301 ggctgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg
      361 cgagctgcac gctgccgtcc tccacgttgt ggcggatctt gaagttcacc ttgatgccgt
      421 tcttctgctt gacggccatg atatagatgt tgtggctgtt gaagttgtac tccagcttgt
      481 gccccaggat gttgccgtcc tccttgaagt cgacgccctt cagctcgatg cggttcacta
      541 gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta ggtgccgtcg tccttgaaga
      601 agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct
      661 tcatgtggtc ggggtagcgg gcgaagcact gcacgccgtg gctcagggtg gtcacgaggg
      721 tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt
      781 tggtggcatc gccctcgccc tcgcccctca cgctgaactt gtggccgttt acgtcgccgt
      841 ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccctt gctcaccata
      901 gagccaccac cggancctcc gccggtacct ccaccgtact cgtcaatncc aanggcatcg
      961 gtaaacatct gcttaaactc gaantcggcc atatccaaaa nccnccntaa aggggcggaa
     1021 atctgggggg gtaaaatccc cggaacccgg ggnaatcccc gtcccccaaa catntcnaaa
     1081 atcnaaaatc gtctancgcg tcggca
//
