LOCUS       sequence-99187-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L4440
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..18
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        42..60
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     118..134
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     118..134
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(204..220)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(205..221)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(246..264)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(274..291)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(274..290)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(283..305)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      432..860
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(519..538)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     729..750
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 agcgagtcag tgagcgagga agcaacctgg cttatcgaaa ttaatacgac tcactatagg
       61 gagaccggca gatctgatat catcgatgaa ttcgagctcc accgcggtgg cggccgctct
      121 agaactagtg gatccaccgg ttccatggct agccacgtga cgcgtggatc ccccgggctg
      181 caggaattcg atatcaagct tatcgatacc gtcgacctcg agggggggcc cggtacccaa
      241 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
      301 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
      361 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
      421 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
      481 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      541 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      601 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      661 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      721 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      781 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      841 acaaaaattt aacgcgaatt ttaacaaaat
//
