LOCUS       sequence-99189-e        1118 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Hartmann C, Tabin CJ
  TITLE     Wnt-14 plays a pivotal role in inducing synovial joint formation in 
            the developing appendicular skeleton.
  JOURNAL   Cell. 2001 Feb 9. 104(3):341-51.
  PUBMED    11239392
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2001 
            Feb 9. 104(3):341-51."
COMMENT     Sequence Label: pGEM cCD44rel (CT#690)
FEATURES             Location/Qualifiers
     source          1..1118
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        22..40
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     22..39
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      611..620
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(650..669)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(651..669)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(676..693)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(676..692)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(685..707)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(920..939)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tgaccatgat tacgccaagc tatttaggtg acactataga atactcaagc tatgcatcca
       61 acgcgttggg agctctccca tatggtcgac ctgcaggcgg ccgcgaattc actagtgatt
      121 gagaagatga tgtgcagcat cctgcaagta ctaggtctac gtccaatata gacagtgatc
      181 agggaccaca caagggagac ggtgaaccta cttcttcccc tggattgtcc acaacaacaa
      241 ctgtaacgtc acaaccaggg acagcccatg taccagaatg gctgatcatc gttgctgccc
      301 tcctggcact ggcattgatc cttgccgtgt gcatcgcggt taacagccag aggagatgtg
      361 ggcagaagaa aaagctagtc attaataatg gcaaaggtgc agtggaggac aggaagacaa
      421 gagaattaaa cggagatgcc agcaagtcac aagaaatggt gcacttggtt cacaaagaac
      481 agtcaaatga ccgaacagga gcatgtgacg aattcctcac cgttgatgaa acacaaaatc
      541 atcaggatgg tgacatgaag agtggagtgt agcggtacga agtcaccaaa taatcgaatt
      601 cccgcggccg ccatggcggc cgggagcatg cgacgtcggg cccaattcgc cctatagtga
      661 gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
      721 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
      781 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc
      841 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
      901 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
      961 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     1021 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagggt gatggttcac
     1081 gtagtgggcc atcgccctga tagacggttt ttcgccct
//
