LOCUS       sequence-9919-ex        3521 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ferguson AA, Fisher AL
  TITLE     Retrofitting ampicillin resistant vectors by recombination for use 
            in generating C. elegans transgenic animals by bombardment.
  JOURNAL   Plasmid. 2009 Sep;62(2):140-5. doi: 10.1016/j.plasmid.2009.06.001. 
            Epub 2009 Jun 9.
  PUBMED    19520111
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.plasmid.2009.06.001"; journalName: "Plasmid"; date: 
            "2009-09"; volume: "62"; issue: "2"; pages: "140-5"
COMMENT     Sequence Label: punc-119c
FEATURES             Location/Qualifiers
     source          1..3521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     64..80
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     65..81
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2244..2260)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2244..2260)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2352..2371)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2363..3178
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             2363..3178
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2436..2455)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ggatcccccg gggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
       61 aactcgaggt cgacggtatc gataagcttc agtaaaagaa gtagaatttt atagtttttt
      121 ttctgtttga aaaattctcc ccatcaatgt tctttcaaat aaatacatca ctaatgcaaa
      181 gtattctata acctcatatc taaattcttc aaaatcttaa catatcttat cattgcttta
      241 agtcaaacgt aacattaaaa aaaatgtttt ggaaaatgtg tcaagtctct caaaattcag
      301 ttttttaaac cactcctata gtcctatagt cctatagtta cccatgaaat ccttatatat
      361 tactgtaaaa tgtttcaaaa accattggca aattgccaga actgaaaatt tccggcaaat
      421 tggggaaccg gcaaattgcc aatttgctga atttgccgga aacggtaatt gccgaaagtt
      481 tttgacacga aaatggcaaa ttgtggtttt aaaatttttt tttttggaaa tttcagaatt
      541 tcaattttaa tcggcaaaac tgtaggcatc ctaagaatgt tcctacatct attttgaaaa
      601 gtaagcgaat tctatgaaaa tgtctaaaga aaatggggaa acaatttcaa aaaggcacag
      661 tttcaatggt ttccgaatta tactaaatcc ctctaaaaac ttccggcaaa ttgatatccg
      721 taaaagagca aatccgcatt tttgccgaaa attaaaattt ccgacaaatc ggcaaaccgg
      781 caatttggcg aaatttgccg gaacgattgc cgcccacccc tgttccagag gttcaaactg
      841 gtagcaaagc tcaaaatttc tcaaattctc caattttttt ttgaattttg gcagtgtacc
      901 aaaatgacat tcagtcatat tggtttatta tagatttatt tagataaaat cctaaatgat
      961 tctaccttta aagatgccca ctttaaaagt aatgactcaa acttcaaatt gctctaagat
     1021 tctattgaat taccatcttt tcctctcatt ttctctcact gtctatttca tcacaaattc
     1081 atccctctct cctctcttct ctctccctct ctctctcttt ctctttgctc atcatctgtc
     1141 attttgtccg ttcctctctc tgcgccctca gcgttcccca cactctctcg cttctctttt
     1201 cctagacgtc ttcttttttc atcttcttca gcctttttcg ccattttcca tctctgtcaa
     1261 tcattacgga cgacccccat taatttttgg atgtgctctg cgagcaagag cacacggaac
     1321 tcatttattt tttgttggaa tttctttttc attttagtta gttaaaagct acaacagcat
     1381 atatgaaggc agagcaacaa caacaatcga tcgcacccgg ctcggcaacc ttcccgtctc
     1441 agatgccccg acccccaccg gtaaccgaac aggctataac caccgaggcg gagcttctcg
     1501 cgaaaaatca aattacacca aatgatgtgc tagctcttcc aggaatcact caaggattcc
     1561 tatgctcccc atcggcaaac gtctataaca ttgagttcac caaattccaa atccgtgacc
     1621 tcgacacgga acacgtgctc ttcgaaattg caaaaccgga aaatgagacg gaagagaatc
     1681 tgcaggcgca agccgaatcg gcaagatatg tccgatatcg atttgcgccg aattttctga
     1741 aattaaagac ggtcggcgcg acggtcgagt tcaaggtcgg cgatgtgccg atcacacatt
     1801 ttcgaatgat tgaacggcac tttttcaagg atcgtttatt gaaatgcttt gattttgaat
     1861 ttggattctg tatgccaaat tcgagaaaca actgtgaaca tatctatgaa tttccacaac
     1921 tttcacaaca actcatggac gacatgatca ataatccaaa cgagacccgc tccgatagct
     1981 tctattttgt ggagaataag ctcgtaatgc acaataaggc cgactactcg tatgatgcat
     2041 aatttcccgc cgatggaata aaactaataa tttttctcgc aatttataga aatcatgtga
     2101 ttttcctacc gctaaattta aaatttccta atttttcact atatttctca caaaatgcat
     2161 tccgaaaact ctcatttttc agcttttcaa tacaaatccc aataaatgat ttttcaaata
     2221 taaaaaaacg aattctgcag cccgatccac tagttctaga attgtgtctc aaaatctctg
     2281 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
     2341 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctcgaggcc
     2401 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     2461 cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt
     2521 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
     2581 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
     2641 tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata
     2701 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
     2761 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca
     2821 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
     2881 gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt
     2941 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
     3001 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
     3061 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
     3121 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
     3181 tagagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gacccccggg
     3241 ggatccgaag atcagcagtt caacctgttg atagtacgta ctaagctctc atgtttcacg
     3301 tactaagctc tcatgtttaa cgtactaagc tctcatgttt aacgaactaa accctcatgg
     3361 ctaacgtact aagctctcat ggctaacgta ctaagctctc atggctaacg tactaagctc
     3421 tcatgtttga acaataaaat taatataaat cagcaactta aatagcctct aaggttttaa
     3481 gttttataag aaaaaaaaga atatataagg cttttaaagc t
//
