LOCUS       sequence-9920-ex        4363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Ferguson AA, Fisher AL
  TITLE     Retrofitting ampicillin resistant vectors by recombination for use 
            in generating C. elegans transgenic animals by bombardment.
  JOURNAL   Plasmid. 2009 Sep;62(2):140-5. doi: 10.1016/j.plasmid.2009.06.001. 
            Epub 2009 Jun 9.
  PUBMED    19520111
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.plasmid.2009.06.001"; journalName: "Plasmid"; date: 
            "2009-09"; volume: "62"; issue: "2"; pages: "140-5"
COMMENT     Sequence Label: punc-119cR
FEATURES             Location/Qualifiers
     source          1..4363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     350..366
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     351..367
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3480..3499)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3491..4306
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             3491..4306
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3564..3583)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ccgggggatc cgaagatcag cagttcaacc tgttgatagt acgtactaag ctctcatgtt
       61 tcacgtacta agctctcatg tttaacgtac taagctctca tgtttaacga actaaaccct
      121 catggctaac gtactaagct ctcatggcta acgtactaag ctctcatggc taacgtacta
      181 agctctcatg tttgaacaat aaaattaata taaatcagca acttaaatag cctctaaggt
      241 tttaagtttt ataagaaaaa aaagaatata taaggctttt aaagctggat cccccggggc
      301 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaact cgaggtcgac
      361 ggtatcgata agcttcagta aaagaagtag aattttatag ttttttttct gtttgaaaaa
      421 ttctccccat caatgttctt tcaaataaat acatcactaa tgcaaagtat tctataacct
      481 catatctaaa ttcttcaaaa tcttaacata tcttatcatt gctttaagtc aaacgtaaca
      541 ttaaaaaaaa tgttttggaa aatgtgtcaa gtctctcaaa attcagtttt ttaaaccact
      601 cctatagtcc tatagtccta tagttaccca tgaaatcctt atatattact gtaaaatgtt
      661 tcaaaaacca ttggcaaatt gccagaactg aaaatttccg gcaaattggg gaaccggcaa
      721 attgccaatt tgctgaattt gccggaaacg gtaattgccg aaagtttttg acacgaaaat
      781 ggcaaattgt ggttttaaaa tttttttttt tggaaatttc agaatttcaa ttttaatcgg
      841 caaaactgta ggcatcctaa gaatgttcct acatctattt tgaaaagtaa gcgaattcta
      901 tgaaaatgtc taaagaaaat ggggaaacaa tttcaaaaag gcacagtttc aatggtttcc
      961 gaattatact aaatccctct aaaaacttcc ggcaaattga tatccgtaaa agagcaaatc
     1021 cgcatttttg ccgaaaatta aaatttccga caaatcggca aaccggcaat ttggcgaaat
     1081 ttgccggaac gattgccgcc cacccctgtt ccagaggttc aaactggtag caaagctcaa
     1141 aatttctcaa attctccaat ttttttttga attttggcag tgtaccaaaa tgacattcag
     1201 tcatattggt ttattataga tttatttaga taaaatccta aatgattcta cctttaaaga
     1261 tgcccacttt aaaagtaatg actcaaactt caaattgctc taagattcta ttgaattacc
     1321 atcttttcct ctcattttct ctcactgtct atttcatcac aaattcatcc ctctctcctc
     1381 tcttctctct ccctctctct ctctttctct ttgctcatca tctgtcattt tgtccgttcc
     1441 tctctctgcg ccctcagcgt tccccacact ctctcgcttc tcttttccta gacgtcttct
     1501 tttttcatct tcttcagcct ttttcgccat tttccatctc tgtcaatcat tacggacgac
     1561 ccccattaat ttttggatgt gctctgcgag caagagcaca cggaactcat ttattttttg
     1621 ttggaatttc tttttcattt tagttagtta aaagctacaa cagcatatat gaaggcagag
     1681 caacaacaac aatcgatcgc acccggctcg gcaaccttcc cgtctcagat gccccgaccc
     1741 ccaccggtaa ccgaacaggc tataaccacc gaggcggagc ttctcgcgaa aaatcaaatt
     1801 acaccaaatg atgtgctagc tcttccagga atcactcaag gattcctatg ctccccatcg
     1861 gcaaacgtct ataacattga gttcaccaaa ttccaaatcc gtgacctcga cacggaacac
     1921 gtgctcttcg aaattgcaaa accggaaaat gagacggaag agaatctgca ggcgcaagcc
     1981 gaatcggcaa gatatgtccg atatcgattt gcgccgaatt ttctgaaatt aaagacggtc
     2041 ggcgcgacgg tcgagttcaa ggtcggcgat gtgccgatca cacattttcg aatgattgaa
     2101 cggcactttt tcaaggatcg tttattgaaa tgctttgatt ttgaatttgg attctgtatg
     2161 ccaaattcga gaaacaactg tgaacatatc tatgaatttc cacaactttc acaacaactc
     2221 atggacgaca tgatcaataa tccaaacgag acccgctccg atagcttcta ttttgtggag
     2281 aataagctcg taatgcacaa taaggccgac tactcgtatg atgcattggt ctcaaagggt
     2341 gaagaagata acatggcaat tattaaagag tttatgcgtt tcaaggtgca tatggaggga
     2401 tctgtcaatg ggcatgagtt tgaaattgaa ggtgaaggag aaggccgacc atatgaggga
     2461 acacaaaccg caaaactaaa ggtaagttta aacatatata tactaactaa ccctgattat
     2521 ttaaattttc aggtaactaa aggcggacca ttaccattcg cctgggacat cctctctcca
     2581 cagttcatgt atggaagtaa agcttatgtt aaacatccgg cagatatacc agattatttg
     2641 aaactttcat tcccggaggg ttttaagtgg gaacgcgtaa tgaattttga agacggagga
     2701 gttgttacag tgacgcaaga ctcaaggtaa gtttaaacag ttcggtacta actaaccata
     2761 catatttaaa ttttcagcct ccaagatgga gaatttattt ataaagtcaa acttcgagga
     2821 acgaatttcc cctcggatgg acctgttatg cagaagaaga ctatgggatg ggaagcttca
     2881 agtgaaagaa tgtaccctga agacggtgct cttaagggag agattaaaca acgtcttaaa
     2941 ttgaaagatg gaggacatta cgatgctgag gtaagtttaa acatgatttt actaactaac
     3001 taatctgatt taaattttca ggtgaagaca acttacaaag ccaaaaaacc agttcagctg
     3061 ccaggagcgt acaatgttaa tattaaactg gatatcacct cccacaacga ggattacact
     3121 atcgttgagc aatatgaaag agctgaaggg cggcactcga caggtggcat ggatgaattg
     3181 tataagacta gttaatttcc cgccgatgga ataaaactaa taatttttct cgcaatttat
     3241 agaaatcatg tgattttcct accgctaaat ttaaaatttc ctaatttttc actatatttc
     3301 tcacaaaatg cattccgaaa actctcattt ttcagctttt caatacaaat cccaataaat
     3361 gatttttcaa atataaaaaa actctagaat tgtgtctcaa aatctctgat gttacattgc
     3421 acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac
     3481 aaggggtgtt atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc
     3541 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     3601 tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     3661 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     3721 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     3781 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     3841 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     3901 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     3961 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     4021 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     4081 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     4141 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     4201 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     4261 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcta gagatcgctg
     4321 agataggtgc ctcactgatt aagcattggt aactgtcaga ccc
//
