LOCUS       sequence-99206-e        1115 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NOTE: A new version of this plasmid is now available. See Addgene 
            plasmid 62988..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F
  TITLE     Genome engineering using the CRISPR-Cas9 system.
  JOURNAL   Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. 
            Epub 2013 Oct 24.
  PUBMED    24157548
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2013.143"; journalName: "Nat Protoc"; date: 
            "2013-11"; volume: "8"; issue: "11"; pages: "2281-308"
COMMENT     Sequence Label: pSpCas9(BB)-2A-Puro (PX459)
FEATURES             Location/Qualifiers
     source          1..1115
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(52..99)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 gacgtcaccg catgttagca gacttcctct gccctctcca ctgccgaatt cctttttctt
       61 ttttgcctgg ccggcctttt tcgtggccgc cggccttttg tcgcctccca gctgagacag
      121 gtcgatccgt gtctcgtaca ggccggtgat gctctggtgg atcagggtgg cgtccagcac
      181 ctctttggtg ctggtgtacc tcttccggtc gatggtggtg tcaaagtact tgaaggcggc
      241 aggggctccc agattggtca gggtaaacag gtggatgata ttctcggcct gctctctgat
      301 gggcttatcc cggtgcttgt tgtaggcgga cagcactttg tccagattag cgtcggccag
      361 gatcactctc ttggagaact cgctgatctg ctcgatgatc tcgtccaggt agtgcttgtg
      421 ctgttccaca aacagctgtt tctgctcatt atcctcgggg gagcccttca gcttctcata
      481 gtggctggcc aggtacagga agttcacata tttggagggc agggccagtt cgtttccctt
      541 ctgcagttcg ccggcagagg ccagcattct cttccggccg ttttccagct cgaacaggga
      601 gtacttaggc agcttgatga tcaggtcctt tttcacttct ttgtagccct tggcttccag
      661 aaagtcgatg ggattcttct cgaagctgct tctttccatg atggtgatcc ccagcagctc
      721 tttcacactc ttcagtttct tggacttgcc cttttccact ttggccacca ccagcacaga
      781 ataggccacg gtggggctgt cgaagccgcc gtacttctta gggtcccagt ccttctttct
      841 ggcgatcagc ttatcgctgt tcctcttggg caggatagac tctttgctga agccgcctgt
      901 ctgcacctcg gtctttttca cgatattcac ttggggcatg ctcagcactt tccgcacggt
      961 ggcaaaatcc cggcccttat cccacacgat ctccccggtt tcgccgtttg tctcgatcag
     1021 aggccgcttc cggatctcgc cgttggccag ggtaatctcg gtcttgaaaa agttcatgat
     1081 gttgctgtag aagaagtact tggcggtagc cttgc
//
