LOCUS       sequence-99214-e       15586 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI
            to EcoRI; 1.4kb), can be replaced w mir30 based hairpin of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15586)
  AUTHORS   Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE, 
            Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi 
            RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF, 
            Elledge SJ
  TITLE     The pINDUCER lentiviral toolkit for inducible RNA interference in 
            vitro and in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 
            10.1073/pnas.1019736108. Epub 2011 Feb 9.
  PUBMED    21307310
REFERENCE   2  (bases 1 to 15586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019736108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-03-1- 1"; volume: "108"; issue: "9"; pages: "3665-70"
COMMENT     Sequence Label: pINDUCER13 (miR-LUP)
FEATURES             Location/Qualifiers
     source          1..15586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    497..730
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             915..959
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1257..1374
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1439..1813)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      1810..1819
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1823..1841)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1833..1880)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    join(1950..1968,1969..1991,1992..2010,2011..2033,2034..2052,
                     2053..2075,2076..2094,2095..2117,2118..2136,2137..2159,
                     2160..2178,2179..2201,2202..2220)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2253..2291
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2288..2312
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2382..2391
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2388..4040
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2454..2472)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     ncRNA           4090..4183
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     4184..4200
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4221..4238
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             4239..4262
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     regulatory      4272..4281
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5557..5577
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     ncRNA           5601..5713
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        complement(5791..5809)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        5827..7038
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6875..6894
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    7045..7078
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(7085..7702,7703..7708,7709..7744,7745..7747,7748..7783,
                     7784..7786,7787..7822,7823..7831)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     protein_bind    complement(7832..7865)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7881..8453
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8048..8065)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8275..8294
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8456..9058
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     8955..8975
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9080..9668
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9133..9153)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9551..9562)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9875..10108
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10209..10226)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10215..10439
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10485..10913
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10572..10591)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10782..10803
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10922..10942)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10927..11256
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11107..11242
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11169..11188
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    12079..12200
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12249..12265)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12249..12265)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12262..12284)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12273..12289
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12297..12303,12304..12321,12322..12327))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12342..12363
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12480..12497)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12651..13239)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12731..12750)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13410..14201,14202..14270))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRMDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14033..14052
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14271..14375)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    14423..14557
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             14725..15358
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ccgagtaata ccgggggaga ttagatcgcg atgggaaaaa ttcggttaag gccaggggga
       61 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
      121 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
      181 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
      241 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
      301 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac
      361 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
      421 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
      481 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
      541 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
      601 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
      661 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
      721 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
      781 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
      841 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
      901 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
      961 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     1021 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     1081 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     1141 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     1201 acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt
     1261 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     1321 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     1381 tattacaggg acagcagaga tccagtttgg ttagtaccgg gcccgctcta gtccggaatc
     1441 agtcctgctc ctcggccacg aagtgcacgc agttgccggc cgggtcgcgc agggcgaact
     1501 cccgccccca cggctgctcg ccgatctcgg tcatggccgg cccggaggcg tcccggaagt
     1561 tcgtggacac gacctccgac cactcggcgt acagctcgtc caggccgcgc acccacaccc
     1621 aggccagggt gttgtccggc accacctggt cctggaccgc gctgatgaac agggtcacgt
     1681 cgtcccggac cacaccggcg aagtcgtcct ccacgaagtc ccgggagaac ccgagccggt
     1741 cggtccagaa ctcgaccgct ccggcgacgt cgcgcgcggt gagcaccgga acggcactgg
     1801 tcaacttggc catggtggcc ctcctatagt gagtcgtatt atactatgcc gatatactat
     1861 gccgatgatt aattgtcaac acgtgctgca ggtccgaggt tctaggctag cttaaaggaa
     1921 ccaattcagt cgactggatc ctttaccact ccctatcagt gatagagaaa agtgaaagtc
     1981 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     2041 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     2101 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     2161 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2221 aaagtgaaag tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg gaggcctata
     2281 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2341 acctccatag aagacaccgg gaccgatcca gcctaccggt cgccaccatg gaagacgcca
     2401 aaaacataaa gaaaggcccg gcgccattct atccgctgga agatggaacc gctggagagc
     2461 aactgcataa ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg
     2521 cacatatcga ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag
     2581 aagctatgaa acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact
     2641 ctcttcaatt ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg
     2701 cgaacgacat ttataatgaa cgtgaattgc tcaacagtat gggcatttcg cagcctaccg
     2761 tggtgttcgt ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa
     2821 tcatccaaaa aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt
     2881 acacgttcgt cacatctcat ctacctcccg gttttaatga atacgatttt gtgccagagt
     2941 ccttcgatag ggacaagaca attgcactga tcatgaactc ctctggatct actggtctgc
     3001 ctaaaggtgt cgctctgcct catagaactg cctgcgtgag attctcgcat gccagagatc
     3061 ctatttttgg caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc
     3121 acggttttgg aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa
     3181 tgtatagatt tgaagaagag ctgtttctga ggagccttca ggattacaag attcaaagtg
     3241 cgctgctggt gccaacccta ttctccttct tcgccaaaag cactctgatt gacaaatacg
     3301 atttatctaa tttacacgaa attgcttctg gtggcgctcc cctctctaag gaagtcgggg
     3361 aagcggttgc caagaggttc catctgccag gtatcaggca aggatatggg ctcactgaga
     3421 ctacatcagc tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag
     3481 ttgttccatt ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta
     3541 atcaaagagg cgaactgtgt gtgagaggtc ctatgattat gtccggttat gtaaacaatc
     3601 cggaagcgac caacgccttg attgacaagg atggatggct acattctgga gacatagctt
     3661 actgggacga agacgaacac ttcttcatcg ttgaccgcct gaagtctctg attaagtaca
     3721 aaggctatca ggtggctccc gctgaattgg aatccatctt gctccaacac cccaacatct
     3781 tcgacgcagg tgtcgcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg
     3841 ttgttttgga gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc
     3901 aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag
     3961 gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg
     4021 gcggaaagat cgccgtgtaa gcggccgcaa gccttgttaa gtgctcgctt cggcagcaca
     4081 tatactatgt tgaatgaggc ttcagtactt tacagaatcg ttgcctgcac atcttggaaa
     4141 cacttgctgg gattacttct tcaggttaac ccaacagaag gctcgaggtc gacggtatcg
     4201 ataagcttga cgtccatatg ctggtacccc gtggaagtct ggaagttctc ttccaggggc
     4261 ccgaaggaga cgccaccatg ggtctagata gggataacag ggtaatggta ccggatcgat
     4321 cccccaactt ccaggcccgc catagtgatt tgatttgcca gcgcatcgct atcaatcgcc
     4381 gggttcaccc attcgcgtaa ccacagttca ctgaatttca taatctattc ctgccttatt
     4441 taaactgttt gaggaaacgc agatcgtttt cgaagaatga acgcaggtcg gtgacgccgt
     4501 aacgcaacat agtcagacgc tccatcccca tcccgaagcc gaaaccagag taaacttccg
     4561 ggtcgatgcc aacgttacgc aacacgttcg gatgcaccat cccgcagccc agcacttcca
     4621 gccatttacc gtttttaccc atgacgtcca cttctgcaga aggttcggta aacgggaagt
     4681 aggaaggacg gaagcgaatc tgcaaatctt cctcaaagaa gttacgcagg aagtcgtgca
     4741 gcgtgccttt caggttggta aagctgatgt tggtatcaac aatcagacct tccatctgat
     4801 ggaacatcgg cgtgtgagtc tggtcgtagt cgttacgata aacacggcca ggcgcgatga
     4861 tacgaatcgg tggctgctgg gctttcatgg tgcggatctg tacgccagag gtctgggtac
     4921 gcagcaggcg ggtagtgtca aaccagaaag tgtcgtggtc agcgcgcgcc gggtggtgac
     4981 caggaatgtt cagagcatcg aagttatgat aatcgtcttc gatttccggc ccggttgcca
     5041 cggtaaagcc aagctcaccg aagaaacttt cgatacggtc gatggtacgg gtaaccggat
     5101 gcagaccgcc gttttcaatg cgacgacctg gcagagagac atcaatcgtt tccgccgcca
     5161 gacgcgcatt cagtgcagcg ctttccagtt ccgctttacg cgcattcagc gcctgctgaa
     5221 cctgctcttt cgcttcgttg ataaccgcac cagctgccgg acgctcttct ggcggcagct
     5281 cacgcagggt cgtcatctga agggttaagt gccctttttt acccaaatat tcgacgcgca
     5341 cattatctaa cgcggcaaca tctgacgcct ggctaatggc cgccttcgca ctggcaacca
     5401 gttctgcgag atgtgacatg gttttcctca ttgtgtcagt ggtgacactg gttcgttgga
     5461 cttagagcct atcccatcag gctattttac ttgccatttt ggtccccgat accgtcgacc
     5521 gcggattacc ctgttatccc tagatctcac aattctgaga atttgtattt tcagggtgat
     5581 ctccgtggat ctattacgaa ttcaaggggc tactttagga gcaattatct tgtttactaa
     5641 aactgaatac cttgctatct ctttgataca tttttacaaa gctgaattaa aatggtataa
     5701 attaaatcac ttttttcaat tggaagacgc ctgtcaagga caccgacgtg gagtcgcccc
     5761 gcgggtatca gggccttttg acaggccaat cctatagtga gtcgtattaa attacgcgta
     5821 agatctggcc tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc ctcacggcga
     5881 gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc cggacgctca
     5941 ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag cagaaggaca
     6001 ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag agcggaacag
     6061 gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg ggcggtgaac
     6121 gccgatgatt atataaggac gcgccgggtg tggcacagct agttccgtcg cagccgggat
     6181 ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca cttggtgagt agcgggctgc
     6241 tgggctggcc ggggctttcg tggccgccgg gccgctcggt gggacggaag cgtgtggaga
     6301 gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg ggggttgggg
     6361 ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt gtgaggcggg
     6421 ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc aaggtcttga
     6481 ggccttcgct aatgcgggaa agctcttatt cgggtgagat gggctggggc accatctggg
     6541 gaccctgacg tgaagtttgt cactgactgg agaactcggt ttgtcgtctg ttgcgggggc
     6601 ggcagttatg gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc gcgccctcgt
     6661 cgtgtcgtga cgtcacccgt tctgttggct tataatgcag ggtggggcca cctgccggta
     6721 ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt cgggcctagg gtaggctctc
     6781 ctgaatcgac aggcgccgga cctctggtga ggggagggat aagtgaggcg tcagtttctt
     6841 tggtcggttt tatgtaccta tcttcttaag tagctgaagc tccggttttg aactatgcgc
     6901 tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac cttttgaaat gtaatcattt
     6961 gggtcaatat gtaattttca gtgttagact agtaaattgt ccgctaaatt ctggccgttt
     7021 ttggcttttt tgttagacgg atccataact tcgtataatg tatgctatac gaagttatgc
     7081 caccatgtct agactggaca agagcaaagt cataaacgga gctctggaat tactcaatgg
     7141 tgtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc
     7201 taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat
     7261 gctggacagg catcataccc acttctgccc cctggaaggc gagtcatggc aagactttct
     7321 gcggaacaac gccaagtcat accgctgtgc tctcctctca catcgcgacg gggctaaagt
     7381 gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt
     7441 cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca
     7501 ctttacactg ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga
     7561 gacacctacc accgattcta tgcccccact tctgagacaa gcaattgagc tgttcgaccg
     7621 gcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa
     7681 acagctaaag tgcgaaagcg gcgggccgac cgacgccctt gacgattttg acttagacat
     7741 gctcccagcc gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga
     7801 cgattttgac cttgacatgc tccccgggta aataacttcg tatagcatac attatacgaa
     7861 gttatggcgc gccaattccg cccctctccc tccccccccc ctaacgttac tggccgaagc
     7921 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     7981 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     8041 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     8101 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     8161 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     8221 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     8281 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     8341 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     8401 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgata ataccatggc
     8461 caccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
     8521 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
     8581 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     8641 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     8701 cgtcgaagcg ggggcggtgt tcgccgagat cggctcgcgc atggccgagt tgagcggttc
     8761 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     8821 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     8881 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgct ggggtgcccg ccttcctgga
     8941 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     9001 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgatt
     9061 aattaagcgc gtctggaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     9121 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     9181 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     9241 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     9301 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     9361 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     9421 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     9481 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     9541 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     9601 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     9661 ctccccgcct ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt
     9721 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     9781 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
     9841 gatcttagcc actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga
     9901 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     9961 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
    10021 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
    10081 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
    10141 ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg tttaaacccg
    10201 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
    10261 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
    10321 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
    10381 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
    10441 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
    10501 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
    10561 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
    10621 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
    10681 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
    10741 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
    10801 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
    10861 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
    10921 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
    10981 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
    11041 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
    11101 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
    11161 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
    11221 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
    11281 attttcggat ctgatcagca cgtgatgaaa aagcctgaac tcaccgcgac gtgaccgcaa
    11341 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
    11401 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
    11461 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
    11521 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
    11581 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
    11641 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
    11701 ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
    11761 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
    11821 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
    11881 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca
    11941 aaggaatagc acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
    12001 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
    12061 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    12121 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    12181 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
    12241 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
    12301 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
    12361 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
    12421 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
    12481 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
    12541 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
    12601 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    12661 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    12721 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    12781 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    12841 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    12901 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    12961 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    13021 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    13081 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    13141 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    13201 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    13261 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    13321 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    13381 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    13441 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    13501 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    13561 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    13621 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    13681 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    13741 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    13801 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    13861 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    13921 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    13981 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtccatacg ggataatacc
    14041 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    14101 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    14161 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    14221 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    14281 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    14341 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    14401 gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag
    14461 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    14521 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    14581 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
    14641 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
    14701 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
    14761 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
    14821 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
    14881 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
    14941 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
    15001 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
    15061 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
    15121 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
    15181 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
    15241 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
    15301 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
    15361 gccgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
    15421 ctcggcttgc tgaagcgcgc acggcaagag gcgaagggcg gcgactggtg agtacgccaa
    15481 aaattttgac tagcggaagg ctagaaggaa gaagatggtg ccagagcgtc agtattaagc
    15541 gggggaaaat tagatcgcga agggaaaaaa ttcggttaag gccagg
//
