LOCUS       sequence-99216-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-H2B-18
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             83..460
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      509..518
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(660..678)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcca ccatgccaga gccagcgaag tctgctcccg ccccgaaaaa
      121 gggctccaag aaggcggtga ctaaggcgca gaagaaaggc ggcaagaagc gcaagcgcag
      181 ccgcaaggag agctattcca tctatgtgta caaggttctg aagcaggtcc accctgacac
      241 cggcatttcg tccaaggcca tgggcatcat gaattcgttt gtgaacgaca ttttcgagcg
      301 catcgcaggt gaggcttccc gcctggcgca ttacaacaag cgctcgacca tcacctccag
      361 ggagatccag acggccgtgc gcctgctgct gcctggggag ttggccaagc acgccgtgtc
      421 cgagggtact aaggccatca ccaagtacac cagcgctaag gatcacggtc acggtactgg
      481 ttctactggt tctggttctt ctccggtcgc caccatggtg agcaagggcg aggaggataa
      541 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
      601 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
      661 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      721 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
      781 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
      841 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
      901 gcgcggcacc aac
//
