LOCUS       sequence-99219-e       15687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI
            to EcoRI;1.4kb),can be replaced w mir30 based hairpin of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15687)
  AUTHORS   Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE, 
            Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi 
            RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF, 
            Elledge SJ
  TITLE     The pINDUCER lentiviral toolkit for inducible RNA interference in 
            vitro and in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 
            10.1073/pnas.1019736108. Epub 2011 Feb 9.
  PUBMED    21307310
REFERENCE   2  (bases 1 to 15687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019736108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-03-1- 1"; volume: "108"; issue: "9"; pages: "3665-70"
COMMENT     Sequence Label: pINDUCER12 (miR-RUL)
FEATURES             Location/Qualifiers
     source          1..15687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    497..730
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             915..959
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1257..1374
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1439..1813)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      1810..1819
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1823..1841)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1833..1880)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    join(1950..1968,1969..1991,1992..2010,2011..2033,2034..2052,
                     2053..2075,2076..2094,2095..2117,2118..2136,2137..2159,
                     2160..2178,2179..2201,2202..2220)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2253..2291
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2288..2312
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2388..3083
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     ncRNA           3134..3227
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     3228..3244
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3265..3282
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             3283..3306
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     regulatory      3316..3325
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4601..4621
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     ncRNA           4645..4757
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        complement(4835..4853)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        4871..6081
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5918..5937
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    6088..6121
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(6128..6745,6746..6751,6752..6787,6788..6790,6791..6826,
                     6827..6829,6830..6865,6866..6874)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     protein_bind    complement(6875..6908)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6924..7496
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7091..7108)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7318..7337
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7499..9151
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(7565..7583)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     misc_feature    9181..9769
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9234..9254)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9652..9663)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9976..10209
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10310..10327)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10316..10540
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10586..11014
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10673..10692)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10883..10904
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11023..11043)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11028..11357
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11208..11343
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11270..11289
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    12180..12301
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12350..12366)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12350..12366)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12363..12385)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12374..12390
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12398..12404,12405..12422,12423..12428))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12443..12464
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12581..12598)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12752..13340)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12832..12851)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13511..14302,14303..14371))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRMDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14134..14153
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14372..14476)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    14524..14658
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             14826..15459
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ccgagtaata ccgggggaga ttagatcgcg atgggaaaaa ttcggttaag gccaggggga
       61 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
      121 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
      181 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
      241 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
      301 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac
      361 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
      421 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
      481 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
      541 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
      601 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
      661 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
      721 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
      781 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
      841 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
      901 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
      961 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     1021 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     1081 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     1141 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     1201 acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt
     1261 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     1321 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     1381 tattacaggg acagcagaga tccagtttgg ttagtaccgg gcccgctcta gtccggaatc
     1441 agtcctgctc ctcggccacg aagtgcacgc agttgccggc cgggtcgcgc agggcgaact
     1501 cccgccccca cggctgctcg ccgatctcgg tcatggccgg cccggaggcg tcccggaagt
     1561 tcgtggacac gacctccgac cactcggcgt acagctcgtc caggccgcgc acccacaccc
     1621 aggccagggt gttgtccggc accacctggt cctggaccgc gctgatgaac agggtcacgt
     1681 cgtcccggac cacaccggcg aagtcgtcct ccacgaagtc ccgggagaac ccgagccggt
     1741 cggtccagaa ctcgaccgct ccggcgacgt cgcgcgcggt gagcaccgga acggcactgg
     1801 tcaacttggc catggtggcc ctcctatagt gagtcgtatt atactatgcc gatatactat
     1861 gccgatgatt aattgtcaac acgtgctgca ggtccgaggt tctaggctag cttaaaggaa
     1921 ccaattcagt cgactggatc ctttaccact ccctatcagt gatagagaaa agtgaaagtc
     1981 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     2041 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     2101 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     2161 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2221 aaagtgaaag tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg gaggcctata
     2281 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2341 acctccatag aagacaccgg gaccgatcca gcctaccggt cgccaccatg agcgagctga
     2401 tcaaggagaa catgcacatg aagctgtaca tggagggcac cgtgaacaac caccacttca
     2461 agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg aagatcaagg
     2521 tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctaccagc ttcatgtacg
     2581 gcagcaaagc cttcatcaac cacacccagg gcatccccga cttctttaag cagtccttcc
     2641 ctgagggctt cacatgggag agaatcacca catacgaaga cgggggcgtg ctgaccgcta
     2701 cccaggacac cagcttccag aacggctgca tcatctacaa cgtcaagatc aacggggtga
     2761 acttcccatc caacggccct gtgatgcaga agaaaacacg cggctgggag gccaacaccg
     2821 agatgctgta ccccgctgac ggcggcctga gaggccacag ccagatggcc ctgaagctcg
     2881 tgggcggggg ctacctgcac tgctccttca agaccacata cagatccaag aaacccgcta
     2941 agaacctcaa gatgcccggc ttccacttcg tggaccacag actggaaaga atcaaggagg
     3001 ccgacaaaga gacctacgtc gagcagcacg agatggctgt ggccaagtac tgcgacctcc
     3061 ctagcaaact ggggcacaga tgatgcggcc gcaagccttg ttaagtgctc gcttcggcag
     3121 cacatatact atgttgaatg aggcttcagt actttacaga atcgttgcct gcacatcttg
     3181 gaaacacttg ctgggattac ttcttcaggt taacccaaca gaaggctcga ggtcgacggt
     3241 atcgataagc ttgacgtcca tatgctggta ccccgtggaa gtctggaagt tctcttccag
     3301 gggcccgaag gagacgccac catgggtcta gatagggata acagggtaat ggtaccggat
     3361 cgatccccca acttccaggc ccgccatagt gatttgattt gccagcgcat cgctatcaat
     3421 cgccgggttc acccattcgc gtaaccacag ttcactgaat ttcataatct attcctgcct
     3481 tatttaaact gtttgaggaa acgcagatcg ttttcgaaga atgaacgcag gtcggtgacg
     3541 ccgtaacgca acatagtcag acgctccatc cccatcccga agccgaaacc agagtaaact
     3601 tccgggtcga tgccaacgtt acgcaacacg ttcggatgca ccatcccgca gcccagcact
     3661 tccagccatt taccgttttt acccatgacg tccacttctg cagaaggttc ggtaaacggg
     3721 aagtaggaag gacggaagcg aatctgcaaa tcttcctcaa agaagttacg caggaagtcg
     3781 tgcagcgtgc ctttcaggtt ggtaaagctg atgttggtat caacaatcag accttccatc
     3841 tgatggaaca tcggcgtgtg agtctggtcg tagtcgttac gataaacacg gccaggcgcg
     3901 atgatacgaa tcggtggctg ctgggctttc atggtgcgga tctgtacgcc agaggtctgg
     3961 gtacgcagca ggcgggtagt gtcaaaccag aaagtgtcgt ggtcagcgcg cgccgggtgg
     4021 tgaccaggaa tgttcagagc atcgaagtta tgataatcgt cttcgatttc cggcccggtt
     4081 gccacggtaa agccaagctc accgaagaaa ctttcgatac ggtcgatggt acgggtaacc
     4141 ggatgcagac cgccgttttc aatgcgacga cctggcagag agacatcaat cgtttccgcc
     4201 gccagacgcg cattcagtgc agcgctttcc agttccgctt tacgcgcatt cagcgcctgc
     4261 tgaacctgct ctttcgcttc gttgataacc gcaccagctg ccggacgctc ttctggcggc
     4321 agctcacgca gggtcgtcat ctgaagggtt aagtgccctt ttttacccaa atattcgacg
     4381 cgcacattat ctaacgcggc aacatctgac gcctggctaa tggccgcctt cgcactggca
     4441 accagttctg cgagatgtga catggttttc ctcattgtgt cagtggtgac actggttcgt
     4501 tggacttaga gcctatccca tcaggctatt ttacttgcca ttttggtccc cgataccgtc
     4561 gaccgcggat taccctgtta tccctagatc tcacaattct gagaatttgt attttcaggg
     4621 tgatctccgt ggatctatta cgaattcaag gggctacttt aggagcaatt atcttgttta
     4681 ctaaaactga ataccttgct atctctttga tacattttta caaagctgaa ttaaaatggt
     4741 ataaattaaa tcactttttt caattggaag acgcctgtca aggacaccga cgtggagtcg
     4801 ccccgcgggt atcagggcct tttgacaggc caatcctata gtgagtcgta ttaaattacg
     4861 cgtaagatct ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg
     4921 gcgagcgctg ccacgtcaga cgaagggcgc agcgagcgtc ctgatcctcc gcccggacgc
     4981 tcaggacagc ggcccgctgc tcataagact cggccttaga accccagtat cagcagaagg
     5041 acattttagg acgggacttg ggtgactcta gggcactggt tttctttcca gagagcggaa
     5101 caggcgagga aaagtagtcc cttctcggcg attctgcgga gggatctccg tggggcggtg
     5161 aacgccgatg attatataag gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg
     5221 gatttgggtc gcggttcttg tttgtggatc gctgtgatcg tcacttggtg agtagcgggc
     5281 tgctgggctg gccggggctt tcgtggccgc cgggccgctc ggtgggacgg aagcgtgtgg
     5341 agagaccgcc aagggctgta gtctgggtcc gcgagcaagg ttgccctgaa ctgggggttg
     5401 gggggagcgc agcaaaatgg cggctgttcc cgagtcttga atggaagacg cttgtgaggc
     5461 gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg gcggcaagaa cccaaggtct
     5521 tgaggccttc gctaatgcgg gaaagctctt attcgggtga gatgggctgg ggcaccatct
     5581 ggggaccctg acgtgaagtt tgtcactgac tggagaactc ggtttgtcgt ctgttgcggg
     5641 ggcggcagtt atggcggtgc cgttgggcag tgcacccgta cctttgggag cgcgcgccct
     5701 cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg cagggtgggg ccacctgccg
     5761 gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg gttcgggcct agggtaggct
     5821 ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg gataagtgag gcgtcagttt
     5881 ctttggtcgg ttttatgtac ctatcttctt aagtagctga agctccggtt ttgaactatg
     5941 cgctcggggt tggcgagtgt gttttgtgaa gttttttagg caccttttga aatgtaatca
     6001 tttgggtcaa tatgtaattt tcagtgttag actagtaaat tgtccgctaa attctggccg
     6061 tttttggctt ttttgttaga cggatccata acttcgtata atgtatgcta tacgaagtta
     6121 tgccaccatg tctagactgg acaagagcaa agtcataaac ggagctctgg aattactcaa
     6181 tggtgtcggt atcgaaggcc tgacgacaag gaaactcgct caaaagctgg gagttgagca
     6241 gcctaccctg tactggcacg tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga
     6301 gatgctggac aggcatcata cccacttctg ccccctggaa ggcgagtcat ggcaagactt
     6361 tctgcggaac aacgccaagt cataccgctg tgctctcctc tcacatcgcg acggggctaa
     6421 agtgcatctc ggcacccgcc caacagagaa acagtacgaa accctggaaa atcagctcgc
     6481 gttcctgtgt cagcaaggct tctccctgga gaacgcactg tacgctctgt ccgccgtggg
     6541 ccactttaca ctgggctgcg tattggagga acaggagcat caagtagcaa aagaggaaag
     6601 agagacacct accaccgatt ctatgccccc acttctgaga caagcaattg agctgttcga
     6661 ccggcaggga gccgaacctg ccttcctttt cggcctggaa ctaatcatat gtggcctgga
     6721 gaaacagcta aagtgcgaaa gcggcgggcc gaccgacgcc cttgacgatt ttgacttaga
     6781 catgctccca gccgatgccc ttgacgactt tgaccttgat atgctgcctg ctgacgctct
     6841 tgacgatttt gaccttgaca tgctccccgg gtaaataact tcgtatagca tacattatac
     6901 gaagttatgg cgcgccaatt ccgcccctct ccctcccccc cccctaacgt tactggccga
     6961 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     7021 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     7081 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     7141 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     7201 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     7261 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     7321 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     7381 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     7441 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg ataataccat
     7501 ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatccgctgg aagatggaac
     7561 cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc
     7621 ttttacagat gcacatatcg aggtggacat cacttacgct gagtacttcg aaatgtccgt
     7681 tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg
     7741 cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc
     7801 agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta tgggcatttc
     7861 gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa
     7921 aaagctccca atcatccaaa aaattattat catggattct aaaacggatt accagggatt
     7981 tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt
     8041 tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact cctctggatc
     8101 tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga gattctcgca
     8161 tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt
     8221 tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg
     8281 agtcgtctta atgtatagat ttgaagaaga gctgtttctg aggagccttc aggattacaa
     8341 gattcaaagt gcgctgctgg tgccaaccct attctccttc ttcgccaaaa gcactctgat
     8401 tgacaaatac gatttatcta atttacacga aattgcttct ggtggcgctc ccctctctaa
     8461 ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg
     8521 gctcactgag actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc
     8581 ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac
     8641 gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt cctatgatta tgtccggtta
     8701 tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg
     8761 agacatagct tactgggacg aagacgaaca cttcttcatc gttgaccgcc tgaagtctct
     8821 gattaagtac aaaggctatc aggtggctcc cgctgaattg gaatccatct tgctccaaca
     8881 ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc
     8941 cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta
     9001 cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga
     9061 agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa
     9121 ggccaagaag ggcggaaaga tcgccgtgta agcggccgct taattaagcg cgtctggaac
     9181 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     9241 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     9301 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     9361 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     9421 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     9481 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     9541 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     9601 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     9661 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     9721 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat
     9781 tctgcagtcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
     9841 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     9901 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     9961 aagaaaagag gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
    10021 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
    10081 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
    10141 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
    10201 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
    10261 gaatatcaga gagtgagagg ccttgacatt gtttaaaccc gctgatcagc ctcgactgtg
    10321 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
    10381 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
    10441 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
    10501 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
    10561 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
    10621 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
    10681 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
    10741 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
    10801 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
    10861 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
    10921 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
    10981 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
    11041 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
    11101 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
    11161 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
    11221 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
    11281 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
    11341 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
    11401 acgtgatgaa aaagcctgaa ctcaccgcga cgtgaccgca aggaatcggt caatacacta
    11461 catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga
    11521 tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg
    11581 aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga
    11641 cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc
    11701 aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga
    11761 cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata
    11821 tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg
    11881 cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc
    11941 gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg
    12001 ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag cacgtgctac
    12061 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
    12121 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
    12181 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    12241 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    12301 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
    12361 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
    12421 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
    12481 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
    12541 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
    12601 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
    12661 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
    12721 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
    12781 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
    12841 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    12901 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
    12961 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    13021 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    13081 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    13141 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
    13201 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    13261 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    13321 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    13381 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    13441 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    13501 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    13561 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    13621 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    13681 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    13741 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    13801 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    13861 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    13921 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    13981 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    14041 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    14101 gaccgagttg ctcttgcccg gcgtccatac gggataatac cgcgccacat agcagaactt
    14161 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    14221 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    14281 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    14341 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    14401 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    14461 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
    14521 atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
    14581 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    14641 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
    14701 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
    14761 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
    14821 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
    14881 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
    14941 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
    15001 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
    15061 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
    15121 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
    15181 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
    15241 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
    15301 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
    15361 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
    15421 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tgccgcccga acagggactt
    15481 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
    15541 cacggcaaga ggcgaagggc ggcgactggt gagtacgcca aaaattttga ctagcggaag
    15601 gctagaagga agaagatggt gccagagcgt cagtattaag cgggggaaaa ttagatcgcg
    15661 aagggaaaaa attcggttaa ggccagg
//
