LOCUS       sequence-99233-e        1118 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and 
            cloning backbone for sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F
  TITLE     Genome engineering using the CRISPR-Cas9 system.
  JOURNAL   Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. 
            Epub 2013 Oct 24.
  PUBMED    24157548
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2013.143"; journalName: "Nat Protoc"; date: 
            "2013-11"; volume: "8"; issue: "11"; pages: "2281-308"
COMMENT     Sequence Label: pSpCas9n(BB)-2A-GFP (PX461)
FEATURES             Location/Qualifiers
     source          1..1118
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggccttatcc agttcgctca ggccgcctct ctcggccttg gtcagattgt cgaactttct
       61 ctgggtaatc agcttggcgt tcagcagctg ccgccagtag ttcttcatct tcttcacgac
      121 ctcttcggag ggcacgttgt cgctcttgcc ccggttcttg tcgcttctgg tcagcacctt
      181 gttgtcgatg gagtcgtcct tcagaaagct ctgaggcacg atatggtcca catcgtagtc
      241 ggacagccgg ttgatgtcca gttcctggtc cacgtacata tcccgcccat tctgcaggta
      301 gtacaggtac agcttctcgt tctgcagctg ggtgttttcc acggggtgtt ctttcaggat
      361 ctggctgccc agctctttga tgccctcttc gatccgcttc attctctcgc ggctgttctt
      421 ctgtcccttc tgggtggtct ggttctctct ggccatttcg atcacgatgt tctcgggctt
      481 gtgccggccc atcactttca cgagctcgtc caccaccttc actgtctgca ggatgccctt
      541 cttaatggcg gggctgccgg ccagattggc aatgtgctcg tgcaggctat cgccctggcc
      601 ggacacctgg gctttctgga tgtcctcttt aaaggtcagg ctgtcgtcgt ggatcagctg
      661 catgaagttt ctgttggcga agccgtcgga cttcaggaaa tccaggattg tcttgccgga
      721 ctgcttgtcc cggatgccgt tgatcagctt ccggctcagc ctgccccagc cggtgtatct
      781 ccgccgcttc agctgcttca tcactttgtc gtcgaacagg tgggcatagg ttttcagccg
      841 ttcctcgatc atctctctgt cctcaaacag tgtcagggtc agcacgatat cttccagaat
      901 gtcctcgttt tcctcattgt ccaggaagtc cttgtccttg ataattttca gcagatcgtg
      961 gtatgtgccc agggaggcgt tgaaccgatc ttccacgccg gagatttcca cggagtcgaa
     1021 gcactcgatt ttcttgaagt agtcctcttt cagctgcttc acggtcactt tccggttggt
     1081 cttgaacagc aggtccacga tggccttttt ctgctcgc
//
