LOCUS       sequence-99259-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express VWF A1-GPIba ReaLiSM construct for single molecule study.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Springer
  TITLE     Springer lab ReaLiSM plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: ReaLiSM A1-43-GPIba+2cys
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(62..79)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(80..100)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     regulatory      802..811
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tctcaccagt ggtcccgctg gagctggatc cggagctgga gctgccgctg gagctggatc
       61 catggtgatg gtgatggtgg ccttgaaaat aaagattctc cccggtacca ggaggggctt
      121 caggggcaag gtcacagagg tagctaacga tctcgtccct ttgctgctcc agctcatcca
      181 cactgctcag cacgaaggcc ttgttctcag gggcctgctt ctcgatgagg cggatctgct
      241 tgaggttggc atggggccca atgcccaccg ggatcacaat gaccttcttc ttcttcaggc
      301 cctggacgta gcggacaaag ttccgggaca tccgttgggg ctcctggctg gccatcagga
      361 gcagggcgat gcgggaggct tcagggcggt cgatcttgct gaagatttgg aacagtgtgt
      421 atttcaagac ctcgctggtg gaggccacct ggctgcccgc atacttcacc tggctggcaa
      481 tgcgccgcag ctctgatggt cgcttccggt ccttgagccc gatgtaggcg tgggagccgt
      541 cgtggtactc caccacggcc acgcggaccc acttctggga gatgcgcagc cgctccatca
      601 tgtccaccac aaaggccttc agcacttcaa actcagcctc ggacagcctg gaggagccat
      661 ccagcaggaa gaccaggtcc agtagcctgc tgcagtagaa atcgtgcaac ggcggttccg
      721 agatgtctcc ggagcacttc cccaacaccc cactgaacca gagcaagagc aaccccagca
      781 attgagcagg aactctcata tccatggtgg cgtacgtcga cgctagcgaa ttcggcctgt
      841 cacg
//
