LOCUS       sequence-99283-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for human MLK1 E179K mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Fawdar S, Trotter EW, Li Y, Stephenson NL, Hanke F, Marusiak AA, 
            Edwards ZC, Ientile S, Waszkowycz B, Miller CJ, Brognard J
  TITLE     Targeted genetic dependency screen facilitates identification of 
            actionable mutations in FGFR4, MAP3K9, and PAK5 in lung cancer.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jul 23;110(30):12426-31. doi: 
            10.1073/pnas.1305207110. Epub 2013 Jul 8.
  PUBMED    23836671
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1305207110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-07-23- 23"; volume: "110"; issue: "30"; pages: 
            "12426-31"
COMMENT     Sequence Label: pDONR221 MLK1 E179K
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    57..81
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa
       61 ctttgtacaa aaaagcaggc ttcgagccct ccagagcgct tctcggctgc ctagcgagcg
      121 ccgccgctgc cgccccgccg ggggaggatg gagcaggggc cggggccgag gaggaggagg
      181 aggaggagga ggaggcggcg gcggcggtgg gccccgggga gctgggctgc gacgcgccgc
      241 tgccctactg gacggccgtg ttcgagtacg aggcggcggg cgaggacgag ctgaccctgc
      301 ggctgggcga cgtggtggag gtgctgtcca aggactcgca ggtgtccggc gacgagggct
      361 ggtggaccgg gcagctgaac cagcgggtgg gcatcttccc cagcaactac gtgaccccgc
      421 gcagcgcctt ctccagccgc tgccagcccg gcggcgagga ccccagttgc tacccgccca
      481 ttcagttgtt agaaattgat tttgcggagc tcaccttgga agagattatt ggcatcgggg
      541 gctttgggaa ggtctatcgt gctttctgga taggggatga ggttgctgtg aaagcagctc
      601 gccacgaccc tgataaggac atcagccaga ccatagagaa tgttcgccaa gaggccaagc
      661 tcttcgccat gctgaagcac cccaacatca ttgccctaag aggggtatgt ctgaaggagc
      721 ccaacctctg cttggtcatg gagtttgctc gtggaggacc tttgaataga gtgttatct
//
