LOCUS       sequence-99285-e         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for human MLK1 E179K mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Fawdar S, Trotter EW, Li Y, Stephenson NL, Hanke F, Marusiak AA, 
            Edwards ZC, Ientile S, Waszkowycz B, Miller CJ, Brognard J
  TITLE     Targeted genetic dependency screen facilitates identification of 
            actionable mutations in FGFR4, MAP3K9, and PAK5 in lung cancer.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jul 23;110(30):12426-31. doi: 
            10.1073/pnas.1305207110. Epub 2013 Jul 8.
  PUBMED    23836671
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1305207110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-07-23- 23"; volume: "110"; issue: "30"; pages: 
            "12426-31"
COMMENT     Sequence Label: pDONR221 MLK1 E179K
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct
       61 ttcttataat gccaactttg tacaagaaag ctgggtccta agaccagaac tcctgctgga
      121 tctcataagg ggcaggcctg tgtgtgttca gttccgctct gcacagcggc acggtgctgt
      181 cctgactctg cccctctgca tccaggtcca ggagcgtccg ctcagtcggg ggcagcggcc
      241 ctgcctggaa cgggagcagg gctggaagtc caggccgctc ctctacagtg ctgctactgc
      301 tagcaaagca ggagtccagg ttgctgggcg tctctgtgct ggagctgttg gctggggagg
      361 tgctgcgggc atggctgggg gacacaaacc accaagggtc cagccgttgc cggttggcag
      421 aaggacgcgg ccgaggcaga aactccagag tcttgggtct ctccaaggta gagtcctgct
      481 gagtgttcca gcgccttggg gttgggggga agaccacatt ggggtcaggg agacggggga
      541 attcacctgg gtctcggctg ggactggggg ttttcaacat tcctgctcca ggactggggc
      601 tcaacccatt gctggagggg ctcctgctgg ctaggagagt ctctggctta agggccccat
      661 cagaaggagt ccgccggtga ctgctgggct gcgagggggt ggtgagggtg acatgggtgg
      721 gagtcagaga ttggttagga tctcgtttga agcgctctac tcggacattg acca
//
