LOCUS       sequence-99290-e         514 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Part of Multibow set of constructs--each FP gene is initially not 
            expressed and then adopts a permanent ON or OFF status upon 
            Cre-mediated recombination. Encodes membrane bound EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Xiong F, Obholzer ND, Noche RR, Megason SG
  TITLE     Multibow: digital spectral barcodes for cell tracing.
  JOURNAL   PLoS One. 2015 May 26;10(5):e0127822. doi: 
            10.1371/journal.pone.0127822. eCollection 2015.
  PUBMED    26010570
REFERENCE   2  (bases 1 to 514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-05-26"; volume: "10(5):e0127822. doi"; pages: " 
            10.1371/journal.pone.0127822. eCollection 2015"
COMMENT     Sequence Label: pMTB-Multibow-mG
FEATURES             Location/Qualifiers
     source          1..514
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(34..67)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(75..108)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(117..150)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      164..173
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttttgcagga tccgccacca tgactagctc gagataactt cgtatagtat accttatacg
       61 aagttatcga gctcataact tcgtatagga tactttatac gaagttataa ctcgagataa
      121 cttcgtatag catacattat acgaagttat accggcagat ctcgccacca tggtgagtgt
      181 gattaaacca gagatgaaga tgaagtactt catggacggc tccgtcaatg ggcatgagtt
      241 cacagttgaa ggtgaaggca caggcaaacc ttacgaggga catcaagaga tgacactacg
      301 cgtcacaatg gccaagggcg ggccaatgcc tttctcgttt gacttagtgt cacacacgtt
      361 ctgttacggc cacagacctt ttactaaata tccagaagag ataccagact atttcaaaca
      421 agcatttcct gaaggcctgt catgggaaag gtcgttgcag ttcgtgggct gggaggcctc
      481 ctccgagcgg atgtaccccg aggacggcgc cctg
//
