LOCUS       sequence-99297-e         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ""CoinFLP-LexGAD/Gal4"" - Expresses LexGAD or Gal4 from actin 
            promoter downsteam of transcriptional stop. Recombination by FLP 
            excises FRT-FRT pair to express LexGAD, or FRT3-FRT3 pair to express
            Gal4..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Bosch JA, Tran NH, Hariharan IK
  TITLE     CoinFLP: a system for efficient mosaic screening and for visualizing
            clonal boundaries in Drosophila.
  JOURNAL   Development. 2015 Feb 1;142(3):597-606. doi: 10.1242/dev.114603.
  PUBMED    25605786
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2015-02-1- 1"; volume: "142"; 
            issue: "3"; pages: "597-606"
COMMENT     Sequence Label: pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    125..158
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tagatgcatc tcaaaaaaat ggtgggcata atagtgttgt ttatatatat caaaaataac
       61 aactataata ataagaatac atttaattta gaaaatgctt ggatttcact ggaactaggc
      121 tagcataact tcgtataatg tatgctatac gaagttatgc tagcggatct cgctgcctgt
      181 tatgtggccc acaaaccaag acacgtttta tggccattaa agctggctga tcgtcgccaa
      241 acaccaaata cataatgaat atgtacacat tcgagaaaga agcgatcaaa gaagcgtctt
      301 cgggcggagt aggagaatgc ggaggagaag gagaacgagc tgatctagta tctctccaca
      361 atccaatgcc aactgaccaa ctggccatat tcggagcaat ttgaagccaa tttccatcgc
      421 ctggcgatcg ctccattctt ggctatatgt ttttcaccgt tacccggggc cattttcaaa
      481 gactcgtcgg caagataaga ttgtgtcact cgctgtctct cttcatttgt cgaagaatgc
      541 tgaggaattt cgcgatgacg tcggcgagta ttttgaagaa tgagaataat ttgtatttat
      601 acgaaaatca gttagtggaa ttttctacaa aaacatgtta tctatagata attttgttgc
      661 aaaatatgtt gactatgaca aagattgtat gtatatacct ttaatgtatt ctcattttct
      721 tatgtattta t
//
