LOCUS       sequence-99301-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ""CoinFLP-LexGAD/Gal4"" - Expresses LexGAD or Gal4 from actin 
            promoter downsteam of transcriptional stop. Recombination by FLP 
            excises FRT-FRT pair to express LexGAD, or FRT3-FRT3 pair to express
            Gal4..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Bosch JA, Tran NH, Hariharan IK
  TITLE     CoinFLP: a system for efficient mosaic screening and for visualizing
            clonal boundaries in Drosophila.
  JOURNAL   Development. 2015 Feb 1;142(3):597-606. doi: 10.1242/dev.114603.
  PUBMED    25605786
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2015-02-1- 1"; volume: "142"; 
            issue: "3"; pages: "597-606"
COMMENT     Sequence Label: pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    629..662
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
ORIGIN
        1 ggcggagcca acttcaacca gagtggtaac attgcggaca gctccttgag cttcacgttt
       61 accaacagct ccaacggtcc aaacttgatc acgactcaga caaactccca ggcattgtcc
      121 cagcccattg ccagctcgaa tgtgcatgat aactttatga ataatgaaat tactgcctcc
      181 aagattgacg acggcaacaa cagcaagccc ctttccccag gctggactga ccagacggcc
      241 tacaatgctt tcggtatcac cactggcatg ttcaacacca caacgatgga cgacgtatat
      301 aactatcttt ttgatgatga ggatactcca ccgaacccaa agaaggagta ggtttaaaca
      361 agcttatcga taccgtcgac taaagccaaa tagaaaatta ttcagttcct ggcttaagtt
      421 tttaaaagtg atattattta tttggttgta accaaccaaa agaatgtaaa taactaatac
      481 ataattatgt tagttttaag ttagcaacaa attgatttta gctatattag ctacttggtt
      541 aataaataga atatatttat ttaaagataa ttgcgttttt attgtcaggg agtgagtttg
      601 cttaaaaact cgtttagatc gcggccgcga agttcctatt cttcaaatag tataggaact
      661 tcagatctta agcaaataaa caagcgcagc tgaacaagct aaacaatctg cagcccaagc
      721 ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta tcgaacaagc atgcgatatt
      781 tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa gtgtctgaag
      841 aacaactggg a
//
