LOCUS       sequence-99302-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ""CoinFLP-LexGAD/Gal4"" - Expresses LexGAD or Gal4 from actin 
            promoter downsteam of transcriptional stop. Recombination by FLP 
            excises FRT-FRT pair to express LexGAD, or FRT3-FRT3 pair to express
            Gal4..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Bosch JA, Tran NH, Hariharan IK
  TITLE     CoinFLP: a system for efficient mosaic screening and for visualizing
            clonal boundaries in Drosophila.
  JOURNAL   Development. 2015 Feb 1;142(3):597-606. doi: 10.1242/dev.114603.
  PUBMED    25605786
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2015-02-1- 1"; volume: "142"; 
            issue: "3"; pages: "597-606"
COMMENT     Sequence Label: pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    120..153
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    174..207
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    497..530
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             547..567
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttaccaaata aggtaaccaa aaactaatgg gaaatccgca ttctttccat tgcagcttac
       61 aggatcgatc cccgggtacg gctagtggat cagcttgcat gcctgcaggt cccctcgagg
      121 aagttcctat tctctagaaa gtataggaac ttccccgcgt accaccagac agcgaagttc
      181 ctattcttca aatagtatag gaacttcgaa ttgactaaag ccaaatagaa aattattcag
      241 ttcctggctt aagtttttaa aagtgatatt atttatttgg ttgtaaccaa ccaaaagaat
      301 gtaaataact aatacataat tatgttagtt ttaagttagc aacaaattga ttttagctat
      361 attagctact tggttaataa atagaatata tttatttaaa gataattgcg tttttattgt
      421 cagggagtga gtttgcttaa aaactcgttt agatccacta gttctagttg cggccggccg
      481 caactagagg atcccggaag ttcctattct ctagaaagta taggaacttc gaattccaaa
      541 atgccaccca agaagaagcg aaaagtagaa gatccaatga aggctctcac ggcccgacaa
      601 caggaagttt ttgatttgat acgggatcat atatcccaaa cgggtatgcc tccgacccgc
      661 gcagagatag cacagcgact gggctttcga tcgcctaacg ccgcggagga gcacttgaag
      721 gcactggccc gcaagggtgt cattgaaatc gtgtccggtg cgagccgcgg aatccggctg
      781 ttgcaggagg aggaagaggg cctgccactg gtgggacgcg tggccgctgg cgagccgctg
      841 ctggcccagc aacacataga gggacacta
//
