LOCUS       sequence-99303-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ""CoinFLP-Gal4"" - Expresses Gal4 from actin promoter downsteam of 
            transcriptional stop. Recombination by FLP excises FRT-FRT pair to 
            express Gal4, or FRT3-FRT3 pair to prevent Gal4 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Bosch JA, Tran NH, Hariharan IK
  TITLE     CoinFLP: a system for efficient mosaic screening and for visualizing
            clonal boundaries in Drosophila.
  JOURNAL   Development. 2015 Feb 1;142(3):597-606. doi: 10.1242/dev.114603.
  PUBMED    25605786
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2015-02-1- 1"; volume: "142"; 
            issue: "3"; pages: "597-606"
COMMENT     Sequence Label: pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..360
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     intron          450..515
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             645..665
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gctttgtttg gtggcgccaa ttttaatcaa agtgggaata ttgctgatag ctcattgtcc
       61 ttcactttca ctaacagtag caacggtccg aacctcataa caactcaaac aaattctcaa
      121 gcgctttcac aaccaattgc ctcctctaac gttcatgata acttcatgaa taatgaaatc
      181 acggctagta aaattgatga tggtaataat tcaaaaccac tgtcacctgg ttggacggac
      241 caaactgcgt ataacgcgtt tggaatcact acagggatgt ttaataccac tacaatggat
      301 gatgtatata actatctatt cgatgatgaa gataccccac caaacccaaa aaaagagtaa
      361 ggtacctcta gaggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca
      421 aactacctac agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg
      481 ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat
      541 gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat
      601 ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa
      661 aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt
      721 ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc
      781 tatacaagaa aattatggaa aaatatttga
//
