LOCUS       sequence-99305-e         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ""CoinFLP-Gal4"" - Expresses Gal4 from actin promoter downsteam of 
            transcriptional stop. Recombination by FLP excises FRT-FRT pair to 
            express Gal4, or FRT3-FRT3 pair to prevent Gal4 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Bosch JA, Tran NH, Hariharan IK
  TITLE     CoinFLP: a system for efficient mosaic screening and for visualizing
            clonal boundaries in Drosophila.
  JOURNAL   Development. 2015 Feb 1;142(3):597-606. doi: 10.1242/dev.114603.
  PUBMED    25605786
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2015-02-1- 1"; volume: "142"; 
            issue: "3"; pages: "597-606"
COMMENT     Sequence Label: pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    138..171
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tttgttcctt gtgtagatgc atctcaaaaa aatggtgggc ataatagtgt tgtttatata
       61 tatcaaaaat aacaactata ataataagaa tacatttaat ttagaaaatg cttggatttc
      121 actggaacta ggctagcata acttcgtata atgtatgcta tacgaagtta tgctagcgga
      181 tctcgctgcc tgttatgtgg cccacaaacc aagacacgtt ttatggccat taaagctggc
      241 tgatcgtcgc caaacaccaa atacataatg aatatgtaca cattcgagaa agaagcgatc
      301 aaagaagcgt cttcgggcgg agtaggagaa tgcggaggag aaggagaacg agctgatcta
      361 gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc aatttgaagc
      421 caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac cgttacccgg
      481 ggccattttc aaagactcgt cggcaagata agattgtgtc actcgctgtc tctcttcatt
      541 tgtcgaagaa tgctgaggaa tttcgcgatg acgtcggcga gtattttgaa gaatgagaat
      601 aatttgtatt tatacgaaaa tcagttagtg gaattttcta caaaaacatg ttatctatag
      661 ataattttgt tgcaaaatat gttgactatg acaaagattg tatgtatata cctttaatgt
      721 attctcattt tcttatgtat ttataatggc aatgatgata ctgatgatat tttaagatga
      781 tgccagacc
//
