LOCUS       sequence-99311-e         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes YFP and NLS tagged beta-actin with sumoylation resistant 
            mutations.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Hofmann WA, Arduini A, Nicol SM, Camacho CJ, Lessard JL, Fuller-Pace
            FV, de Lanerolle P
  TITLE     SUMOylation of nuclear actin.
  JOURNAL   J Cell Biol. 2009 Jul 27;186(2):193-200. doi: 10.1083/jcb.200905016.
  PUBMED    19635839
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2009-07-27- 27"; volume: "186"; 
            issue: "2"; pages: "193-200"
COMMENT     Sequence Label: EYFP-NLS-actin-K68/284R
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(38..40,41..43,44..754)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDLKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(83..104)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(344..363)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     691..712
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgaaccgtca gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct
       61 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      121 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      181 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg
      241 cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      301 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      361 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
      421 catcgacctc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
      481 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
      541 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
      601 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct
      661 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
      721 cgggatcact ctcggcatgg acgagctgta caagcc
//
