LOCUS       sequence-99316-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes MYC tagged beta-actin with a mutation in the NES.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Hofmann WA, Arduini A, Nicol SM, Camacho CJ, Lessard JL, Fuller-Pace
            FV, de Lanerolle P
  TITLE     SUMOylation of nuclear actin.
  JOURNAL   J Cell Biol. 2009 Jul 27;186(2):193-200. doi: 10.1083/jcb.200905016.
  PUBMED    19635839
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2009-07-27- 27"; volume: "186"; 
            issue: "2"; pages: "193-200"
COMMENT     Sequence Label: myc-actin-NES-1
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..25
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6..24
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             51..80
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 agatgtaata cgactcacta tagggcgaat tggccgcggt taccaacatg gaacaaaaac
       61 tcatctcaga agaggatctg aatcgatcca tgaattctag tggatccatg gatgatgata
      121 tcgccgcgct cgtcgtcgac aacggctccg gcatgtgcaa ggccggcttc gcgggcgacg
      181 atgccccccg ggccgtcttc ccctccatcg tggggcgccc caggcaccag ggcgtgatgg
      241 tgggcatggg tcagaaggat tcctatgtgg gcgacgaggc ccagagcaag agaggcatcc
      301 tcaccctgaa gtaccccatc gagcacggca tcgtcaccaa ctgggacgac atggagaaaa
      361 tctggcacca caccttctac aatgagctgc gtgtggctcc cgaggagcac cccgtgctgc
      421 tgaccgaggc ccccctgaac cccaaggcca accgcgagaa gatgacccag atcatgtttg
      481 agaccttcaa caccccagcc atgtacgttg ctatccaggc tgtgctatcc ctgtacgcct
      541 ctggccgtac cactggcatc gtgatggact ccggtgacgg ggtcacccac actgtgccca
      601 tctacgaggg gtatgccctc ccccatgcca tcctgcgtgc ggacgcggct ggccgggacc
      661 tgactgacta cctcatgaag atcctcaccg agcgcggcta cagcttcacc accacggccg
      721 agcgggaaat cgtgcgtgac attaaggaga agctgtgcta cgtcgccctg gacttcgagc
      781 aagagatggc cacggctgct tccagctcct ccctggagaa gagctacgag ctgcctgacg
      841 gccaggtcat caccattggc aatgagcggt tccgctg
//
