LOCUS       sequence-9933-ex       13281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13281)
  AUTHORS   Chang CW, Lai YS, Pawlik KM, Liu K, Sun CW, Li C, Schoeb TR, Townes 
            TM
  TITLE     Polycistronic lentiviral vector for ""hit and run"" reprogramming of
            adult skin fibroblasts to induced pluripotent stem cells.
  JOURNAL   Stem Cells. 2009 May . 27(5):1042-9.
  PUBMED    19415770
REFERENCE   2  (bases 1 to 13281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2009 May . 27(5):1042-9."
COMMENT     Sequence Label: pKP332 (Lenti-OSK1)
FEATURES             Location/Qualifiers
     source          1..13281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             3..636
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    683..808
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1301..1534
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1719..1763
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2121..3298
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2352..3289
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3246..3266
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4412..4468
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5429..5485
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    6935..7052
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7087..7675
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7140..7160)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7558..7569)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7844..7877)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7897..8077
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8102..8120)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8103..8120)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8410..8429)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8643..8662)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8762..8784
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8822..8840)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8908..9012
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9013..9081,9082..9873)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9231..9250)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10044..10632
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10533..10552
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10786..10803
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10873..10893)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10878..11207
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11058..11193
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          12341..12406
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12536..12556
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12981..13115
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13018..13037)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13072..13091
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca
       61 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
      121 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
      181 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
      241 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
      301 agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc
      361 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag
      421 atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
      481 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
      541 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
      601 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
      661 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
      721 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
      781 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
      841 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
      901 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
      961 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1021 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1081 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1141 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     1201 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     1261 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     1321 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     1381 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     1441 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     1501 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     1561 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     1621 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     1681 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     1741 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     1801 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     1861 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     1921 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     1981 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgatgtcga cgataagctt
     2041 tgcaaagatg gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg
     2101 tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
     2161 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc
     2221 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     2281 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     2341 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg
     2401 gcccttgcgt gccttgaatt acttccactg gctgcagtac gtgattcttg atcccgagct
     2461 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
     2521 tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct
     2581 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
     2641 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
     2701 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
     2761 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
     2821 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
     2881 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
     2941 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
     3001 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
     3061 gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg
     3121 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
     3181 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
     3241 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagg
     3301 aatttcgaca tttaaatcca ccatggcggg acacctggct tcggatttcg ccttctcgcc
     3361 ccctccaggt ggtggaggtg atgggccagg ggggccggag ccgggctggg ttgatcctcg
     3421 gacctggcta agcttccaag gccctcctgg agggccagga atcgggccgg gggttgggcc
     3481 aggctctgag gtgtggggga ttcccccatg ccccccgccg tatgagttct gtggggggat
     3541 ggcgtactgt gggccccagg ttggagtggg gctagtgccc caaggcggct tggagacctc
     3601 tcagcctgag ggcgaagcag gagtcggggt ggagagcaac tccgatgggg cctccccgga
     3661 gccctgcacc gtcacccctg gtgccgtgaa gctggagaag gagaagctgg agcaaaaccc
     3721 ggaggagtcc caggacatca aagctctgca gaaagaactc gagcaatttg ccaagctcct
     3781 gaagcagaag aggatcaccc tgggatatac acaggccgat gtggggctca ccctgggggt
     3841 tctatttggg aaggtattca gccaaacgac catctgccgc tttgaggctc tgcagcttag
     3901 cttcaagaac atgtgtaagc tgcggccctt gctgcagaag tgggtggagg aagctgacaa
     3961 caatgaaaat cttcaggaga tatgcaaagc agaaaccctc gtgcaggccc gaaagagaaa
     4021 gcgaaccagt atcgagaacc gagtgagagg caacctggag aatttgttcc tgcagtgccc
     4081 gaaacccaca ctgcagcaga tcagccacat cgcccagcag cttgggctcg agaaggatgt
     4141 ggtccgagtg tggttctgta accggcgcca gaagggcaag cgatcaagca gcgactatgc
     4201 acaacgagag gattttgagg ctgctgggtc tcctttctca gggggaccag tgtcctttcc
     4261 tctggcccca gggccccatt ttggtacccc aggctatggg agccctcact tcactgcact
     4321 gtactcctcg gtccctttcc ctgaggggga agcctttccc cctgtctccg tcaccactct
     4381 gggctctccc atgcattcaa acggatccgg agccacgaac ttctctctgt taaagcaagc
     4441 aggagatgtt gaagaaaacc ccgggcctat gtacaacatg atggagacgg agctgaagcc
     4501 gccgggcccg cagcaaactt cggggggcgg cggcggcaac tccaccgcgg cggcggccgg
     4561 cggcaaccag aaaaacagcc cggaccgcgt caagcggccc atgaatgcct tcatggtgtg
     4621 gtcccgcggg cagcggcgca agatggccca ggagaacccc aagatgcaca actcggagat
     4681 cagcaagcgc ctgggcgccg agtggaaact tttgtcggag acggagaagc ggccgttcat
     4741 cgacgaggct aagcggctgc gagcgctgca catgaaggag cacccggatt ataaataccg
     4801 gccccggcgg aaaaccaaga cgctcatgaa gaaggataag tacacgctgc ccggcgggct
     4861 gctggccccc ggcggcaata gcatggcgag cggggtcggg gtgggcgccg gcctgggcgc
     4921 gggcgtgaac cagcgcatgg acagttacgc gcacatgaac ggctggagca acggcagcta
     4981 cagcatgatg caggaccagc tgggctaccc gcagcacccg ggcctcaatg cgcacggcgc
     5041 agcgcagatg cagcccatgc accgctacga cgtgagcgcc ctgcagtaca actccatgac
     5101 cagctcgcag acctacatga acggctcgcc cacctacagc atgtcctact cgcagcaggg
     5161 cacccctggc atggctcttg gctccatggg ttcggtggtc aagtccgagg ccagctccag
     5221 cccccctgtg gttacctctt cctcccactc cagggcgccc tgccaggccg gggacctccg
     5281 ggacatgatc agcatgtatc tccccggcgc cgaggtgccg gaacccgccg cccccagcag
     5341 acttcacatg tcccagcact accagagcgg cccggtgccc ggcacggcca ttaacggcac
     5401 actgcccctc tcacacatgg gatccggagc cacgaacttc tctctgttaa agcaagcagg
     5461 agatgttgaa gaaaaccccg ggcctatggc tgtcagcgac gcgctgctcc catctttctc
     5521 cacgttcgcg tctggcccgg cgggaaggga gaagacactg cgtcaagcag gtgccccgaa
     5581 taaccgctgg cgggaggagc tctcccacat gaagcgactt cccccagtgc ttcccggccg
     5641 cccctatgac ctggcggcgg cgaccgtggc cacagacctg gagagcggcg gagccggtgc
     5701 ggcttgcggc ggtagcaacc tggcgcccct acctcggaga gagaccgagg agttcaacga
     5761 tctcctggac ctggacttta ttctctccaa ttcgctgacc catcctccgg agtcagtggc
     5821 cgccaccgtg tcctcgtcag cgtcagcctc ctcttcgtcg tcgccgtcga gcagcggccc
     5881 tgccagcgcg ccctccacct gcagcttcac ctatccgatc cgggccggga acgacccggg
     5941 cgtggcgccg ggcggcacgg gcggaggcct cctctatggc agggagtccg ctccccctcc
     6001 gacggctccc ttcaacctgg cggacatcaa cgacgtgagc ccctcgggcg gcttcgtggc
     6061 cgagctcctg cggccagaat tggacccggt gtacattccg ccgcagcagc cgcagccgcc
     6121 aggtggcggg ctgatgggca agttcgtgct gaaggcgtcg ctgagcgccc ctggcagcga
     6181 gtacggcagc ccgtcggtca tcagcgtcag caaaggcagc cctgacggca gccacccggt
     6241 ggtggtggcg ccctacaacg gcgggccgcc gcgcacgtgc cccaagatca agcaggaggc
     6301 ggtctcttcg tgcacccact tgggcgctgg accccctctc agcaatggcc accggccggc
     6361 tgcacacgac ttccccctgg ggcggcagct ccccagcagg actaccccga ccctgggtct
     6421 tgaggaagtg ctgagcagca gggactgtca ccctgccctg ccgcttcctc ccggcttcca
     6481 tccccacccg gggcccaatt acccatcctt cctgcccgat cagatgcagc cgcaagtccc
     6541 gccgctccat taccaagagc tcatgccacc cggttcctgc atgccagagg agcccaagcc
     6601 aaagagggga agacgatcgt ggccccggaa aaggaccgcc acccacactt gtgattacgc
     6661 gggctgcggc aaaacctaca caaagagttc ccatctcaag gcacacctgc gaacccacac
     6721 aggtgagaaa ccttaccact gtgactggga cggctgtgga tggaaattcg cccgctcaga
     6781 tgaactgacc aggcactacc gtaaacacac ggggcaccgc ccgttccagt gccaaaaatg
     6841 cgaccgagca ttttccaggt cggaccacct cgccttacac atgaagaggc atttttaaat
     6901 ttaaatttaa ttaatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg
     6961 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     7021 tacaaaaaca aattacaaaa attcaaaatt ttccgatcac gagactagcc tcgagggaat
     7081 tccgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     7141 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     7201 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     7261 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     7321 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     7381 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     7441 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     7501 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     7561 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     7621 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcgg
     7681 gaattcgctc aagcttcgaa ttaattctgc agagctcggt acctttaaga ccaatgactt
     7741 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     7801 ttcactccca acgaagacaa gatgggatca attcaccatg ggaataactt cgtatagcat
     7861 acattatacg aagttatgct gctttttgct tgtactgggt ctctctggtt agaccagatc
     7921 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7981 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     8041 ctcagaccct tttagtcagt gtggaaaatc tctagcagca tctagaatta attccgtgta
     8101 ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat taattgtagc
     8161 cgcgttctaa cgacaatatg tacaagccta attgtgtagc atctggctta ctgaagcaga
     8221 ccctatcatc tctctcgtaa actgccgtca gagtcggttt ggttggacga accttctgag
     8281 tttctggtaa cgccgtcccg cacccggaaa tggtcagcga accaatcagc agggtcatcg
     8341 ctagccagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
     8401 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
     8461 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
     8521 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
     8581 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaatg gtgcactctc
     8641 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     8701 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     8761 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     8821 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     8881 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     8941 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     9001 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     9061 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     9121 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     9181 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     9241 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     9301 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     9361 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     9421 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     9481 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     9541 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     9601 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     9661 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     9721 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     9781 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     9841 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     9901 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     9961 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    10021 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    10081 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    10141 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
    10201 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    10261 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    10321 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    10381 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga
    10441 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    10501 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    10561 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    10621 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    10681 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    10741 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    10801 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    10861 taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
    10921 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
    10981 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
    11041 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
    11101 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
    11161 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttggacaca
    11221 agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt
    11281 gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat
    11341 ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca
    11401 cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa
    11461 ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc
    11521 ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt
    11581 gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag
    11641 gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt actgcggttg
    11701 cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc
    11761 gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatt gaacagccgt
    11821 gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg
    11881 cactgccggg cgttgttctt tttaacttca ggcgggttac aatagtttcc agtaagtatt
    11941 ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt
    12001 aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg
    12061 cagtcggccc ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg
    12121 tggatctggc gcggcattga cccacgcgaa atcctcgacg tccaggcacg tattgtgatg
    12181 agcgatgccg aacgtaccga cgatgattta tacgatacgg tgattggcta ccgtggcggc
    12241 aactggattt atgagtgggc cccggatctt tgtgaaggaa ccttacttct gtggtgtgac
    12301 ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag
    12361 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg
    12421 aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga
    12481 agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa
    12541 aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag
    12601 tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa
    12661 agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca
    12721 taacagttat aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc
    12781 tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa
    12841 taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg
    12901 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
    12961 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
    13021 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    13081 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
    13141 atcatcccaa acttcccacc ccatacccta ttaccactgc caattaccta gtggtttcat
    13201 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
    13261 atatgtacta caaacttagt a
//
