LOCUS       sequence-99335-e         584 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Part of Multibow set of constructs--each FP gene is initially not 
            expressed and then adopts a permanent ON or OFF status upon 
            Cre-mediated recombination. Encodes cytoplasmic EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Xiong F, Obholzer ND, Noche RR, Megason SG
  TITLE     Multibow: digital spectral barcodes for cell tracing.
  JOURNAL   PLoS One. 2015 May 26;10(5):e0127822. doi: 
            10.1371/journal.pone.0127822. eCollection 2015.
  PUBMED    26010570
REFERENCE   2  (bases 1 to 584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-05-26"; volume: "10(5):e0127822. doi"; pages: " 
            10.1371/journal.pone.0127822. eCollection 2015"
COMMENT     Sequence Label: pMTB-Multibow-G
FEATURES             Location/Qualifiers
     source          1..584
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(32..65)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(73..106)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(115..148)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      162..171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttgcaggatc cgccaccatg actagctcga gataacttcg tatagtatac cttatacgaa
       61 gttatcgagc tcataacttc gtataggata ctttatacga agttataact cgagataact
      121 tcgtatagca tacattatac gaagttatac cggcagatct cgccaccatg gtgagtgtga
      181 ttaaaccaga gatgaagatg aagtacttca tggacggctc cgtcaatggg catgagttca
      241 cagttgaagg tgaaggcaca ggcaaacctt acgagggaca tcaagagatg acactacgcg
      301 tcacaatggc caagggcggg ccaatgcctt tctcgtttga cttagtgtca cacacgttct
      361 gttacggcca cagacctttt actaaatatc cagaagagat accagactat ttcaaacaag
      421 catttcctga aggcctgtca tgggaaaggt cgttgcagtt cgtgggctgg gaggcctcct
      481 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
      541 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctac
//
