LOCUS       sequence-99342-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression empty backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  TITLE     Vectors
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 1435 pSG5L Flag HA
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          64..636
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     644..663
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     691..710
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        691..709
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             740..763
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             776..802
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             776..802
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 agaggctttt ttggaggcct aggcttttgc aaaaagctcc ggatcgatcc tgagaacttc
       61 agggtgagtt tggggaccct tgattgttct ttctttttcg ctattgtaaa attcatgtta
      121 tatggagggg gcaaagtttt cagggtgttg tttagaatgg gaagatgtcc cttgtatcac
      181 catggaccct catgataatt ttgtttcttt cactttctac tctgttgaca accattgtct
      241 cctcttattt tcttttcatt ttctgtaact ttttcgttaa actttagctt gcatttgtaa
      301 cgaattttta aattcacttt tgtttatttg tcagattgta agtactttct ctaatcactt
      361 ttttttcaag gcaatcaggg tatattatat tgtacttcag cacagtttta gagaacaatt
      421 gttataatta aatgataagg tagaatattt ctgcatataa attctggctg gcgtggaaat
      481 attcttattg gtagaaacaa ctacatcctg gtcatcatcc tgcctttctc tttatggtta
      541 caatgatata cactgtttga gatgaggata aaatactctg agtccaaacc gggcccctct
      601 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
      661 gtgctgtctc atcattttgg caaagaattg taatacgact cactataggg cgaattgtcg
      721 acccgggaag cttaccatgg actacaagga cgacgatgac aagctcgatg gaggataccc
      781 ctacgacgtg cccgactacg ccagcggatc cgcgcggatc cgcgaattcc ccgatatcgc
      841 ctcgagtcta gatccagatc ttattaaagc a
//
