LOCUS       sequence-99344-e        1254 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses REX-GECO0.9 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Wu J, Abdelfattah AS, Miraucourt LS, Kutsarova E, Ruangkittisakul A,
            Zhou H, Ballanyi K, Wicks G, Drobizhev M, Rebane A, Ruthazer ES, 
            Campbell RE
  TITLE     A long Stokes shift red fluorescent Ca(2+) indicator protein for 
            two-photon and ratiometric imaging.
  JOURNAL   Nat Commun. 2014 Oct 31;5:5262. doi: 10.1038/ncomms6262.
  PUBMED    25358432
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-10-31"; volume: "5:5262. doi"; pages: " 
            10.1038/ncomms6262"
COMMENT     Sequence Label: CMV-REX-GECO0.9
FEATURES             Location/Qualifiers
     source          1..1254
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..72
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     768..787
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggtcgact catcacgtcg taagtggaat aaggcaggtc acgcagtcag agctataggt
       61 cggctgagct cacgttgggt ttccgagtgg atgtaccccg aggacggcgc cctgaagagc
      121 gtgatcaagg aggggctgag gctgaaggac ggcggccact acgccgccga ggtcaggacc
      181 acctacaagg ccaaaaagcc cgtgcagctg cccggcgcct acatcgtcga catcaagttg
      241 gacatcgtgt cccacaacga ggactacacc atcgtggaac agtgcgaacg cgccgtgggc
      301 cgccacccca ccggcggcat ggtcgagctg tacaagggag gtacaggcgg gagtctggtg
      361 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
      421 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      481 tacgaggcct ttcagaccgc taagctgaag gtgaccaagg gtggccccct gcccttcgcc
      541 tgggacatcc tgtcccttca gttcatgtac ggctccaagg cctacattaa gcacccagcc
      601 gacatccccg actacttcaa gctgtccttc cccgagggct tcaggtggga gcgcgtgatg
      661 atcttcgagg acggcggcat tattcacgtt gaccaggact cctccctgca ggacggcgta
      721 ttcatctaca aggtgaagct gcgcggcacc aacttccccc ccgacggccc cgtaatgcag
      781 aagaagacca tgggctggga gcctacgcgt gaccaactga ctgaagagca gatcgcagag
      841 tttaaagagg ctttctccct atttgacaag gacggggatg ggacgataac aaccaaggag
      901 ctggggacgg tgttgcggtc tctggggcag aaccccacag aagcagagct gcaggacatg
      961 atcaatgaag tagatgccga cggtgacggc acattcgact tccctgagtt cctgacgatg
     1021 atggcaagga aaatgaatga ctcagacagt gaagaggaaa ttagagaagc gttccgcgtg
     1081 tttgataagg acggcaatgg ctacatcggc gcagcagagc ttcgccacgt gatgacagac
     1141 cttggagaga agttaacaga tgaggaggtt gatgaaatga tcagggtagc agacatcgat
     1201 ggggatggtc aggtaaacta cgaagagttt gtacaaatga tgacagcgaa gtag
//
