LOCUS       sequence-99353-e         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast C-terminal tagging vector, which creates gene fusions with 
            GFP-gamma using SpHis5 selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Slubowski CJ, Funk AD, Roesner JM, Paulissen SM, Huang LS
  TITLE     Plasmids for C-terminal tagging in Saccharomyces cerevisiae that 
            contain improved GFP proteins, Envy and Ivy.
  JOURNAL   Yeast. 2015 Jan 22. doi: 10.1002/yea.3065.
  PUBMED    25612242
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Jan 22. doi: 10.1002/yea.3065."
COMMENT     Sequence Label: pFA6a-link-GFP-gamma-SpHis5
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(68..265)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 gatctgatat catcgatgaa ttcgagctcg tttaaactgg atggcggcgt tagtatcgaa
       61 tcgacagcag tatagcgacc agcattcaca tacgattgac gcatgatatt actttctgcg
      121 cacttaactt cgcatctggg cagatgatgt cgaggcgaaa aaaaatataa atcacgctaa
      181 catttgatta aaatagaaca actacaatat aaaaaaacta tacaaatgac aagttcttga
      241 aaacaagaat ctttttattg tcagtactct ttacaacact cccttcgtgc ttgggacttc
      301 agaacttcca gtaagactag tagccgcgcg catggcaaca gccagagatt taaaagcgct
      361 ttcagcacga tgatggtcat tactaccata taagcaggta acatgcaaag taattccagc
      421 tgctaccgaa aaggaatata gtaagtgagg gatcatttca caggacaatt ccccaacctt
      481 ttcacgcttt aatcccaaat cgataacagc atagggccgt cccgacaagt caactacgct
      541 tctagaaaga gcttcgtcaa gtggacaata agcatgtcca aatcttttaa cgccggcaaa
      601 gttactcata gcctgcttga atgcaatacc aagtgcaata gcagtatctt ctgcagtgtg
      661 atgatcatcg atgattaaat cacctcttga gtaaagtcgt aagctccagc ctgcatgttt
      721 agccagtgca tgatacatgt gatccaagaa tccaattcc
//
