LOCUS       sequence-99354-e         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses both fragments of T7 RNAP split at position 179. The 
            N-terminal fragment is driven by PLac while the C-terminal fragment 
            is driven by PBAD. C-terminal fragment has the point mutations 
            R756S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pTSlb-R756K
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(156..183)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 tcgctaacca aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa
       61 gccatgacaa aaacgcgtaa tttcaacacg agccaaaata ataaaaaagc aggattaata
      121 atctggcttt ttatattctc tctctagtat ataaacgcag aaaggcccac ccgaaggtga
      181 gccagtgtga tccaagccag ctcgatatcc gtcggcttga acgaattgtt agacattact
      241 tgtagacgtg ccctacgcgc ttgttgagtt gttcctcaac gtttttcttg aagtgcttag
      301 cttcaaggtc acggatacga ccgaagcgag cctcgtcctc aatggcccga ccgattgcgc
      361 ttgctacagc ctgaacggtt gtattgtcag cactggttag gcaagccaga gtggtcttaa
      421 tggtgatgta cgctacggct tccggcttga tttcttgcag gaactggaag gctgtcgggc
      481 gcttgccgcg cttagctttc acttcctcaa accagtcgtt gatgcgtgca atcatcttag
      541 ggagtagggt agtgatgaga ggcttggcgg cagcgttatc cgcaacctca ccagctttaa
      601 gttgacgctc aaacatcttg cggaagcgtg cttcacccat ctcgtaagac tcatgctcaa
      661 gggccaactg ttcgcgagct aaacgctcac cgtaatggtc agccagagtg ttgaacggga
      721 tagcagccag ttcgatgtca gagaagtcgt tcttagcgat gttaatcgtg ttcatattag
      781 aaaaag
//
