LOCUS       sequence-99356-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses both fragments of T7 RNAP split at position 179. The 
            N-terminal fragment is driven by PLac while the C-terminal fragment 
            is driven by PBAD. C-terminal fragment has the point mutations 
            R756S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pTSlb-R756K
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgcaactctc tactgtttct ccatacccgt ttttttgggc tagcactagt ggagccctat
       61 atataaagat cagggccagg tatgaaagca tttatgcaag ttgtcgaggc tgacatgctc
      121 tctaagggtc tactcggtgg cgaggcgtgg tcttcgtggc ataaggaaga ctctattcat
      181 gtaggagtac gctgcatcga gatgctcatt gagtcaaccg gaatggttag cttacaccgc
      241 caaaatgctg gcgtagtagg tcaagactct gagactatcg aactcgcacc tgaatacgct
      301 gaggctatcg caacccgtgc aggtgcgctg gctggcatct ctccgatgtt ccaaccttgc
      361 gtagttcctc ctaagccgtg gactggcatt actggtggtg gctattgggc taacggtcgt
      421 cgtcctctgg cgctggtgcg tactcacagt aagaaagcac tgatgcgcta cgaagacgtt
      481 tacatgcctg aggtgtacaa agcgattaac attgcgcaaa acaccgcatg gaaaatcaac
      541 aagaaagtcc tagcggtcgc caacgtaatc accaagtgga agcattgtcc ggtcgaggac
      601 atccctgcga ttgagcgtga agaactcccg atgaaaccgg aagacatcga catgaatcct
      661 gaggctctca ccgcgtggaa acgtgctgcc gctgctgtgt accgcaagga caaggctcgc
      721 aagtctcgcc gtatcagcct tgagttcatg cttgagcaag ccaataagtt tgctaaccat
      781 aaggccatct ggttccctta caacatgg
//
