LOCUS       sequence-99357-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses both fragments of T7 RNAP split at position 179. The 
            N-terminal fragment is driven by PLac while the C-terminal fragment 
            is driven by PBAD. C-terminal fragment has the point mutations 
            R756S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pTSlb-R756K
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gatgagaaca ctggtgaaat ctctgagaaa gtcaagctgg gcactaaggc actggctggt
       61 caatggctgg cttacggtgt tactcgcagt gtgactaagc gttcagtcat gacgctggct
      121 tacgggtcca aagagttcgg cttccgtcaa caagtgctgg aagataccat tcagccagct
      181 attgattccg gcaagggtct gatgttcact cagccgaatc aggctgctgg atacatggct
      241 aagctgattt gggaatctgt gagcgtgacg gtggtagctg cggttgaagc aatgaactgg
      301 cttaagtctg ctgctaagct gctggctgct gaggtcaaag ataagaagac tggagagatt
      361 cttcgcaagc gttgcgctgt gcattgggta actcctgatg gtttccctgt gtggcaggaa
      421 tacaagaagc ctattcagac gcgcttgaac ctgatgttcc tcggtcagtt caaattacag
      481 cctaccatta acaccaacaa agatagcgag attgatgcac acaaacagga gtctggtatc
      541 gctcctaact ttgtacacag ccaagacggt agccaccttc gtaagactgt agtgtgggca
      601 cacgagaagt acggaatcga atcttttgca ctgattcacg actccttcgg taccattccg
      661 gctgacgctg cgaacctgtt caaagcagtg cgcgaaacta tggttgacac atatgagtct
      721 tgtgatgtac tggctgattt ctacgaccag ttcgctgacc agttgcacga gtctcaattg
      781 gacaaaatgc cagcacttcc ggctaaaggt aacttgaacc tccgtgacat cttagagtcg
      841 gacttcgcgt tcgcgtaatc tagagtcgac ctgcaggcat gcaagc
//
