LOCUS       sequence-99364-e        1072 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP reporter for CRISPR/CAS9 and gRNA_AAVS1-T2 activities.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Zhang JH, Pandey M, Kahler JF, Loshakov A, Harris B, Dagur PK, Mo Y,
            Simonds WF
  TITLE     Improving the specificity and efficacy of CRISPR/CAS9 and gRNA 
            through target specific DNA reporter.
  JOURNAL   J Biotechnol. 2014 Sep 2. pii: S0168-1656(14)00838-4. doi: 
            10.1016/j.jbiotec.2014.08.033.
  PUBMED    25193712
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Biotechnol. 2014 Sep 2. pii: S0168-1656(14)00838-4. doi: 
            10.1016/j.jbiotec.2014.08.033."
COMMENT     Sequence Label: psAAVS1-T2
FEATURES             Location/Qualifiers
     source          1..1072
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    131..252
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(142..161)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     196..215
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    376..432
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     complement(488..509)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     837..856
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
       61 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
      121 atttacgcgt taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
      181 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
      241 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
      301 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcag
      361 ttatctagat ccggtggatc ccgggcccgc ggtaccgtcg actgcagaat tcgaagcttg
      421 agctcgagat ctgagtccgg ccggacttgt acagctcgtc catgccgaga gtgatcccgg
      481 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca
      541 gggcggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
      601 gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga
      661 tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc
      721 tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
      781 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
      841 tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg
      901 cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc
      961 cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga
     1021 tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg ga
//
