LOCUS       sequence-99366-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast C-terminal tagging vector, which creates gene fusions with 
            GFPEnvy using SpHis5 selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Slubowski CJ, Funk AD, Roesner JM, Paulissen SM, Huang LS
  TITLE     Plasmids for C-terminal tagging in Saccharomyces cerevisiae that 
            contain improved GFP proteins, Envy and Ivy.
  JOURNAL   Yeast. 2015 Jan 22. doi: 10.1002/yea.3065.
  PUBMED    25612242
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Jan 22. doi: 10.1002/yea.3065."
COMMENT     Sequence Label: pFA6a-link-GFPEnvy-SpHis5
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(69..266)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 ggatctgata tcatcgatga attcgagctc gtttaaactg gatggcggcg ttagtatcga
       61 atcgacagca gtatagcgac cagcattcac atacgattga cgcatgatat tactttctgc
      121 gcacttaact tcgcatctgg gcagatgatg tcgaggcgaa aaaaaatata aatcacgcta
      181 acatttgatt aaaatagaac aactacaata taaaaaaact atacaaatga caagttcttg
      241 aaaacaagaa tctttttatt gtcagtactc tttacaacac tcccttcgtg cttgggactt
      301 cagaacttcc agtaagacta gtagccgcgc gcatggcaac agccagagat ttaaaagcgc
      361 tttcagcacg atgatggtca ttactaccat ataagcaggt aacatgcaaa gtaattccag
      421 ctgctaccga aaaggaatat agtaagtgag ggatcatttc acaggacaat tccccaacct
      481 tttcacgctt taatcccaaa tcgataacag catagggccg tcccgacaag tcaactacgc
      541 ttctagaaag agcttcgtca agtggacaat aagcatgtcc aaatctttta acgccggcaa
      601 agttactcat agcctgcttg aatgcaatac caagtgcaat agcagtatct tctgcagtgt
      661 gatgatcatc gatgattaaa tcacctcttg agtaaagtcg taagctccag cctgcatgtt
      721 tagccagtgc atgatacatg tgatccaaga atccaattcc cgtgtctact tggattactt
      781 gttctccctt ttggtttgta tgcttggaag ttataagttc atcaataaa
//
