LOCUS       sequence-99369-e         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses both fragments of T7 RNAP split at position 179. The 
            N-terminal fragment is driven by PLac while the C-terminal fragment 
            is driven by PBAD. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pTSlb-wt
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtcaacaagt gctggaagat accattcagc cagctattga ttccggcaag ggtctgatgt
       61 tcactcagcc gaatcaggct gctggataca tggctaagct gatttgggaa tctgtgagcg
      121 tgacggtggt agctgcggtt gaagcaatga actggcttaa gtctgctgct aagctgctgg
      181 ctgctgaggt caaagataag aagactggag agattcttcg caagcgttgc gctgtgcatt
      241 gggtaactcc tgatggtttc cctgtgtggc aggaatacaa gaagcctatt cagacgcgct
      301 tgaacctgat gttcctcggt cagttccgct tacagcctac cattaacacc aacaaagata
      361 gcgagattga tgcacacaaa caggagtctg gtatcgctcc taactttgta cacagccaag
      421 acggtagcca ccttcgtaag actgtagtgt gggcacacga gaagtacgga atcgaatctt
      481 ttgcactgat tcacgactcc ttcggtacca ttccggctga cgctgcgaac ctgttcaaag
      541 cagtgcgcga aactatggtt gacacatatg agtcttgtga tgtactggct gatttctacg
      601 accagttcgc tgaccagttg cacgagtctc aattggacaa aatgccagca cttccggcta
      661 aaggtaactt gaacctccgt gacatcttag agtcggactt cgcgttcgcg taatctagag
      721 tcga
//
