LOCUS       sequence-9937-ex       10794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10794)
  AUTHORS   Mayr C, Bartel DP
  TITLE     Widespread shortening of 3'UTRs by alternative cleavage and 
            polyadenylation activates oncogenes in cancer cells.
  JOURNAL   Cell. 2009 Aug 21. 138(4):673-84.
  PUBMED    19703394
REFERENCE   2  (bases 1 to 10794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Aug 21. 138(4):673-84."
COMMENT     Sequence Label: pIS1 IMP-1 rc
FEATURES             Location/Qualifiers
     source          1..10794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..759
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     intron          829..961
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     944..963
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1006..1024
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1034..1969
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    8749..8870
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8760..8779)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8814..8833
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        9003..9107
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9108..9176,9177..9968)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9326..9345)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10139..10727
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10628..10647
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agatctaaat gagtcttcgg acctcgcggg ggccgcttaa gcggtggtta gggtttgtct
       61 gacgcggggg gagggggaag gaacgaaaca ctctcattcg gaggcggctc ggggtttggt
      121 cttggtggcc acgggcacgc agaagagcgc cgcgatcctc ttaagcaccc ccccgccctc
      181 cgtggaggcg ggggtttggt cggcgggtgg taactggcgg gccgctgact cgggcgggtc
      241 gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac ccccgggcgg cgccgccgcg
      301 gcggcgacgg gctcgctggg tcctaggctc catggggacc gtatacgtgg acaggctctg
      361 gagcatccgc acgactgcgg tgatattacc ggagaccttc tgcgggacga gccgggtcac
      421 gcggctgacg cggagcgtcc gttgggcgac aaacaccagg acggggcaca ggtacactat
      481 cttgtcaccc ggaggcgcga gggactgcag gagcttcagg gagtggcgca gctgcttcat
      541 ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt
      601 ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac
      661 gcagatgcag tcggggcggc gcggtcccag gtccacttcg catattaagg tgacgcgtgt
      721 ggcctcgaac accgagcgac cctgcagcga cccgcttaaa agcttgattc ttctgacaca
      781 acagtctcga acttaagctg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag
      841 gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc
      901 ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca
      961 ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta
     1021 taggctagcc accatgactt cgaaagttta tgatccagaa caaaggaaac ggatgataac
     1081 tggtccgcag tggtgggcca gatgtaaaca aatgaatgtt cttgattcat ttattaatta
     1141 ttatgattca gaaaaacatg cagaaaatgc tgttattttt ttacatggta acgcggcctc
     1201 ttcttattta tggcgacatg ttgtgccaca tattgagcca gtagcgcggt gtattatacc
     1261 agaccttatt ggtatgggca aatcaggcaa atctggtaat ggttcttata ggttacttga
     1321 tcattacaaa tatcttactg catggtttga acttcttaat ttaccaaaga agatcatttt
     1381 tgtcggccat gattggggtg cttgtttggc atttcattat agctatgagc atcaagataa
     1441 gatcaaagca atagttcacg ctgaaagtgt agtagatgtg attgaatcat gggatgaatg
     1501 gcctgatatt gaagaagata ttgcgttgat caaatctgaa gaaggagaaa aaatggtttt
     1561 ggagaataac ttcttcgtgg aaaccatgtt gccatcaaaa atcatgagaa agttagaacc
     1621 agaagaattt gcagcatatc ttgaaccatt caaagagaaa ggtgaagttc gtcgtccaac
     1681 attatcatgg cctcgtgaaa tcccgttagt aaaaggtggt aaacctgacg ttgtacaaat
     1741 tgttaggaat tataatgctt atctacgtgc aagtgatgat ttaccaaaaa tgtttattga
     1801 atcggaccca ggattctttt ccaatgctat tgttgaaggt gccaagaagt ttcctaatac
     1861 tgaatttgtc aaagtaaaag gtcttcattt ttcgcaagaa gatgcacctg atgaaatggg
     1921 aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat gaacaataat tctaggagct
     1981 ctataccggt ctcgatatca ctactagtgt tctagaccag cccctccctg tcccttcgag
     2041 tccaggacaa caacgggcag aaatcgagag tgtgctctcc ccggcaggcc tgagaatgag
     2101 tgggaatccg ggacacctgg gccgggctgt agatcaggtt tgcccacttg attgagaaag
     2161 atgttccagt gaggaaccct gatctctcag ccccaaacac ccacccaatt ggcccaacac
     2221 tgtctgcccc tcggggtgtc agaaattcta gcgcaaggca cttttaaacg tggattgttt
     2281 aaagaagctc tccaggcccc accaagaggg tggatcacac ctcagtggga cgcaaactca
     2341 aatttccttc aggttttaaa aacatgcaga gaggtgtttt aatcagcctt aaaggatggt
     2401 tcatttgcgg ccgcttgtct ttcaatataa gtttcctttt attttgaaat tgaaacagca
     2461 aaatgaagtg gtttgtggct gaaacaacct tcacgggaaa gaaaactgga gtgttcgttc
     2521 atccatcaaa gaacaaactc caacgtgtga gccagctgcc ccacctcccc caaagcaatg
     2581 aacagattaa aggatgggag gaaggataca atcaaaatcg ggtggtgatg gctggcagat
     2641 ttccagtaaa atacaatttt caaagttgcc gcaattacag aataagctag cactcatatt
     2701 taaaaaaaaa aaaaaaaaaa aaactgtccc aagcagtagg cctccagctc ttcccaattt
     2761 gcactcatca aaatacatcg ttccctcttc tgagtaagtg atgtttcaga actgcttaca
     2821 aatccacttt gcctcagggg aggaagctct tttgattcac tctggttttg gggaccagga
     2881 tcaagatcct ggagaaaagg gagactccaa atggtgatag aggacgttta ttcattatgg
     2941 taacacataa tctgctgaaa ttcattttga acgtgctttt acatagcagt ggctaacata
     3001 aaatctgcac agaaatgtta caagtggctc cccagccccc acagccggga agtgccagtc
     3061 aagatttaag catccccaaa acaaagcctt tattcctggg ggagcttatt ggcaggggag
     3121 gggggacagg ggtgactgaa gtcagctggc tgggagggga gaagggagag aagcacagga
     3181 aggactcact ggcaggaata gggtggggga tggggtgaga caggccaggg gcagactgtg
     3241 aaagctgtta cctgttggaa tagctagcac gttatcttaa acaagtgaag aaaatcgcca
     3301 aagagtgaaa cttatttatg ggggaaaaaa aagcttggga aattaaaaca atccagtggt
     3361 cactggaaaa tgtctccctt ttaaaaaaaa attttttttt ttggtttttt tttttttaat
     3421 tttatttttt ggaaagtatt ctcaaggtaa tatttttttc ctttggaaaa agaaatcttt
     3481 ttccccccct aacctaagca agtggagctg ccactaccta atttatctat tgttttgcta
     3541 agaggtagat aaaacaaaag tgaaatgggg cttctatgag gaggtccatt agagggtggg
     3601 tggggagggg ctgggatcgc ccagtggaac tcccccaact acaaagacgg cctgaatgag
     3661 tccaaggacc gcgccctcag actcccccag ggcaaaggat cctgcttcac ctcttctcct
     3721 tctagagcca actgggcccc cattgaagac tgaaaaaggg cccaatggag gtgtaggatc
     3781 tcaaagaaac cctgttgaag aggtctcagc tctggtttcc ccaggcttcc tgactttggg
     3841 gctgtgtggt gggttccagg ccaagcccca ggtctcactc cagccagccc cctctagaag
     3901 ggaagaggcc attcctttcc tcaccatctg gtccaacaaa gctatagaaa gtgagagagg
     3961 gacaagctgg aggccaaagg ggtgacacat gggcaaggaa agcagaaagt ggggacctca
     4021 tggccagccc tcccagccca atcgatatgg gggggatcct ggcaggcctg cctgttcccc
     4081 aaggacaaac cttttgtggc acaagcctca actcacttgt cctggctggg attcataccc
     4141 ttatgcatga gtccctgccc ttagttccaa tcttactgta aggattgagg tagttaatcc
     4201 atttatactc gtagcatcca tcacctgcgg ttaatcaaaa cacatgctcc cgaggagctt
     4261 cctgctattt cattcaagaa aaaaaatttt ttttcttttg tccatccgga gtcaaccata
     4321 ctaaggcgcc agcaaaggcg ccatcagact gtgccccaaa gcggtgcaca ggaccgaggg
     4381 tcagggctgg ggctctcgcc cctaaatccc ccttcagaca tgaggcctcc tccctgtccc
     4441 gtaccccgat agagaagcct tatgtatcca aaggaaagaa actgcagttt tcagctggtg
     4501 ttactctaag tctgaataca gagaaatgtt ctgcatctaa attgtcgagt atttggaagg
     4561 gaaatggctt ttatgtcccc tggcattgat gaggattttt cttcaatctt cctgcaatta
     4621 ctgtgacagg ctgtgctgtt tcccttctac tggttttcaa gaaacaggca aacagtcgag
     4681 tggtttgaat ggcaaagcca ctaggatctc cccataaaca cttcagggga aagaaaggag
     4741 aacccccacc cctgatgaga ggtgggagga aggggttgcc tagaggcacc agcttctcct
     4801 gtggagcaag aaggctcaag caggccctct ggggctctgt gaggtagtta ctagcactgc
     4861 tggttcccag tttggcagag ggtatgtctg ggttgggagg aggggaaggc acccttgaga
     4921 gctaactgag gcccctcctc actgcccgtc tgccaggcct gaagggaact cggcagtgct
     4981 atctctgcag taggggcagg ggacagagca gtcccacaac agggagctac ctggagcccc
     5041 aaacttcctc tccttcccca aagtgcaacc aggagccgac agaccaattt ctcggctccc
     5101 taccccttgg cttggcaaac accacaccct aatcccccag gagcctctga agggaagggc
     5161 ctcgccctta gctcccagtt tccaggaggc cttcctcctc agacagccag gtatgtgtcg
     5221 aaagctggct attgctttga ttcaggaaca gcctctctca gcctctgtct gtctgtcttt
     5281 ggcacagagg aaaatggaca cagaaccaac tagagcatcc ccctccagtg ttctctgggt
     5341 tttaaatggg gtcttttctg ccaatagtga ggcataaaca aggatagctc ccaactgcct
     5401 ccagaaggag ggggtcagga aaaaggaaaa aagattgata aggactggga ggaggggagg
     5461 aaagtagaaa agggctataa aactccctgt ccttaaaaat ggctctctcc taaccagacc
     5521 cctcacagga gctgagtcca caacccaaac tgcaaaaggc aaccagaccc gagaggggat
     5581 taaagccatt tggggtgtaa ctggcagatg cagcttcaaa gtcagtcagt gaatcaaacc
     5641 attttggtta tgtgtttcaa agaatttaaa aatatatcct aaagagatgg tcctttctta
     5701 aaaatatata tgtagcacac acacacacat atatatctta gagcacagaa gcattttttt
     5761 taagtcactc ttctcaaatc atccaaatct cccaagtgct tttcgcgata gcttgttatc
     5821 tcaccaagga tggggaaatg gaggtgctga gcaggagggc aaggagttgc cctaagacct
     5881 agcctacggg gcacaagagg gcccggccca ccaagacagg tccttcatcc cactaaacct
     5941 taaccaccaa accatgctcc ttttgctttc tggtggtata acttacatga ttttcctgct
     6001 gggcaaatat caacaatgag tggagccttg cctaatccaa gcaagtgaag ttttccagtc
     6061 ccttgccaca cacttcctcg tctcttctgg gagctgaatc agccaacgga aaaggacttg
     6121 ggctcaaggt gtgaagggag caggccccca aggaaactga acactgcaac aatcacattt
     6181 caggtactgt gggatttttt tttcttcctg atgatagtct gaaccagata tcaaagcatg
     6241 cttgctgctg gaaattcgct ttaaattact aaatggccga cgtctgaacg ttatctgctt
     6301 tgttttggga taaagcatat agagccagag atggaaagct gggagaccct ttttatggtt
     6361 gggacaggtg gagaggctta attctactgg cctgatgtta tccctaaatg tttttctgct
     6421 tctattcaat ttgggccaga caattctggg ggctacaagg gaatgtccta gggagaaaat
     6481 gataaggcag gaacctgtcc cagtcacgtc cccatatccc ctgcccctcc ttgccaccag
     6541 ccaccctaaa tcaagaattc cctaagtttg ggtccacttt taaaatcaat cagaccgatc
     6601 taaagaagac agggagacag aggtgggggc agttaactag tatctgtttg ccccttagat
     6661 ctatggatat agattaggaa gaatagcaag acggaactga aatgaaacaa taacaaaaaa
     6721 aaaaaatgaa aagcattgca atgacaaacc aggtggacct ctctgagggc gagagggtgg
     6781 ccttgtcttt tttccacgaa cggaatctca gctcccatat ttttagacag agaaatatag
     6841 gtttttttaa agggtatatt ttttagttat tatttctctc ctcgataaaa cggaggcaat
     6901 attttaaaat atctcaacag caccactggt gggaaaagct ggctgagatg ctctttaacc
     6961 aaaggaggaa agagaggaga ccaagggcca gctggttggt tgttgggcat tgtttaacca
     7021 ctgagagccc ccagctccac taggcctttt aatcctatca ggtaggatat tccagcagcc
     7081 gccagcagct ggggctctag aagtcagaga tcagggccaa acaaggctct gggctctgcc
     7141 cctctgggct ctctcaaacc tgggccttcc ctcttagcac tagcctcctc ctaagggggc
     7201 agcccccaaa gcctttccca ccaggcctgt tctccataga gagttcctaa agggttactg
     7261 gctcatgttg taagaaatta tgaaacataa ctttcttgtt ttttcttttc attaaaaaaa
     7321 taaaatattc aagactatca gcttctcttt tgctttgctt ttctttttta tataaaatat
     7381 cagcaagctg caaaaaaaca aaaatcaaaa aaaaattttt ttaatggggt tggggggaac
     7441 aaaaaggaaa ataattgctg aggtaacttc ataccttgag gtagtttact tcgcacacag
     7501 cattgcctga ctacgcccac atgttgcggt tgtcttgttg ttactgttgt tgcaatgttg
     7561 aaaggagggg aactccacgc atgggctttt ccatatgtag agaggccttc ctcccttccc
     7621 agcacacttg atagcattgt ccagggtagc taaagtgcat tacctggcta atatgcattg
     7681 tcaatcagta tattaggagg agaggcctaa cctcatttaa ctgatggctt ctgtctccca
     7741 caagggctaa acactccatg ggatcttaat taagcctggt aaattaataa attaagctta
     7801 tttacccctt gctgtacaat gctgctggta ttctgaattg ttttttagac acacctttca
     7861 tcatgctgat aatgattcat ggagacagaa gccgtcagtt aaaaattcct ctcagataca
     7921 aacagaagca agtactctcc aatttactga atttgccaga tgatggactt tagctttatc
     7981 gggagctcag ggttgggttc caagtcccac caccaaggta acagtgccgt ggctgggagt
     8041 gccccacaac ccctgcctct aggaaccagc ccttatggga gcacatgggc tggggtgagg
     8101 gcaccgtggc agcctttggg gccactcaag tcctccatgg aaccgagact ttatcaaggg
     8161 gagttagcgt ccccttccca gtgctcatat ccaatcatta aaatagactt taaaaaaata
     8221 taaaatccgc ctgagaacga tgggaatcag ggacagtggt cagcccctac cctgctgagc
     8281 attcaatacc acctatgtgc acctggacat aatgtgaaac ctacacggga gagggacatg
     8341 aatgtggtgg ggaagcccca acagagtggg gaaatttcag ttgtgccaat ttgaagatgg
     8401 tgatctggca tagatcagcc acaagtgcgc tccttttaaa cgtggattat tatgtatttt
     8461 aaccatcttt ctccccattt tcccctcttc ttcattccgt attgtaccta ttggttaggg
     8521 agtgattgga attaatctac agatactgac agttcttttc ccttgagtca atactgtaag
     8581 caaagcaatg cattcgttcg catttttttc tggtagcaat aaagataaaa aaattagtgt
     8641 aaatatagaa agaggccgta aatggaggta ttttaatcaa ttacccaagt agggggaaga
     8701 agattggaaa aacattaagg tcaaggcggc cgcttcgagc agacatgata agatacattg
     8761 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     8821 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     8881 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     8941 aaaacctcta caaatgtggt aaaatcgata aggatccagg tggcactttt cggggaaatg
     9001 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     9061 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     9121 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     9181 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     9241 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     9301 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     9361 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     9421 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     9481 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     9541 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     9601 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     9661 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     9721 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     9781 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     9841 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     9901 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     9961 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
    10021 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
    10081 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    10141 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    10201 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    10261 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
    10321 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    10381 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    10441 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    10501 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    10561 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    10621 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    10681 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    10741 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatggct cgac
//
