LOCUS       sequence-99372-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GeneGuard genomic cassette: for constructing Delta-dapA::RBS A pi, 
            Kis, FRT-kanR-FRT (low-copy support for R6K COR). Will support 
            pSEVAD1K..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Wright O, Delmans M, Stan GB, Ellis T
  TITLE     GeneGuard: a Modular Plasmid System Designed for Biosafety.
  JOURNAL   ACS Synth Biol. 2014 May 13.
  PUBMED    24847673
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 May 13."
COMMENT     Sequence Label: pSEVA12DKR-A
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(382..415)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     RBS             695..703
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             743..751
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      complement(820..847)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt gatcagatct tgatcccctg
       61 cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc
      121 ttaccagagg gcgccccagc tggcaattcc ggttcgcttg ctgtccataa aaccgcccag
      181 tctagctatc gccatgtaag cccactgcaa gctacctgct ttctctttgc gcttgcgttt
      241 tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg tttctgcgga
      301 ctggctttct acgtgttccg cttcctttag cagcccttgc gccctgagtg cttgcggcag
      361 cgtgagcttc aaaagcgctc tgaagttcct atactttcta gagaatagga acttcgaact
      421 gcaggtcgac tcagatttcc tcctgaccag tcgccggtgc atccagccat tctcgttctt
      481 cagctgatat ttcagcattc ggatcacact gtgccagtag ctcagccagt gaatattgcg
      541 ggcgtctgta cggctcaaca atcagccggc cattatcaat gaccatgcca acttcattat
      601 ctgtgcccag agacagcgca ttcagcagtg ccggtgggac ggtcagcata actgagccgc
      661 caaccctctt cagtcgggtg gtatgcatta gaaaacctcc ttagcatgat taagatgttt
      721 cttcaacact cgcatggttg ttacctcctt acctctggtc tccacacatt atacctatag
      781 gttagacttt aagtcaatac tctttttccc gggtataaac gcagaaaggc ccacccgaag
      841 gtgagccagt gtgactctag tagagagcgt tcaccgacaa acaacagata aaacgaaagg
      901 cccagtcttt cgactgagcc tttcg
//
