LOCUS       sequence-99373-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GeneGuard genomic cassette: for constructing Delta-dapA::RBS A pi, 
            Kis, FRT-kanR-FRT (low-copy support for R6K COR). Will support 
            pSEVAD1K..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Wright O, Delmans M, Stan GB, Ellis T
  TITLE     GeneGuard: a Modular Plasmid System Designed for Biosafety.
  JOURNAL   ACS Synth Biol. 2014 May 13.
  PUBMED    24847673
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 May 13."
COMMENT     Sequence Label: pSEVA12DKR-A
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(41..718)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
ORIGIN
        1 gtctttcgac tgagcctttc gttttatttg atgcctttaa ttaagcaccg gtggagtgac
       61 gaccttcagc acgttcgtac tgttcaacga tggtgtagtc ttcgttgtgg gaggtgatgt
      121 ccagtttgat gtcggttttg taagcacccg gcagctgaac cggtttttta gccatgtagg
      181 tggttttaac ttcagcgtcg tagtgaccac cgtctttcag tttcagacgc attttgattt
      241 cacctttcag agcaccgtct tccgggtaca tacgttcggt ggaagcttcc caacccatgg
      301 tttttttctg cataaccgga ccgtcggacg ggaagttggt accacgcagt ttaactttgt
      361 agatgaactc accgtcttgc agggaggagt cctgggtaac ggtaacaaca ccaccgtctt
      421 cgaagttcat aacacgttcc catttgaaac cttccgggaa ggacagtttc aggtagtccg
      481 ggatgtcagc cgggtgttta acgtaagctt tggaaccgta ctggaactgc ggggacagga
      541 tgtcccaagc gaacggcagc ggaccacctt tggtaacttt cagtttagcg gtctgggtac
      601 cttcgtacgg acgaccttca ccttcacctt cgatttcgaa ctcgtgaccg ttaacggaac
      661 cttccatacg aactttgaaa cgcatgaact ctttgataac gtcttcggag gaagccatct
      721 agtattcacc acctttctct agtaacattt attgtacaac acgagcccat ttttgtcaaa
      781 taaaatttaa attatatcaa cgttaataag acgttgtcaa taaaattatt ttgacaaaat
      841 tcctaggcgt cgaaaagtgc tgtgaagcgt tcaattaaat gcggggg
//
