LOCUS       sequence-99375-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GeneGuard genomic cassette: for constructing Delta-dapA::RBS A pi, 
            Kis, FRT-kanR-FRT (low-copy support for R6K COR). Will support 
            pSEVAD1K..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 684 to 718)
  AUTHORS   Wright O, Delmans M, Stan GB, Ellis T
  TITLE     GeneGuard: a Modular Plasmid System Designed for Biosafety.
  JOURNAL   ACS Synth Biol. 2014 May 13.
  PUBMED    24847673
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 May 13."
COMMENT     Sequence Label: pSEVA12DKR-A
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        684..718
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
ORIGIN
        1 acgaactttg aaacgcatga actctttgat aacgtcttcg gaggaagcca tctagtattc
       61 accacctttc tctagtaaca tttattgtac aacacgagcc catttttgtc aaataaaatt
      121 taaattatat caacgttaat aagacgttgt caataaaatt attttgacaa aattcctagg
      181 cgtcgaaaag tgctgtgaag cgttcaatta aatgcgggga gtctgccaca tcgacctgaa
      241 cccagacaga tgctggcatt ggcggacgcg gacgcgccgt cgtgcgcttc atcacgatta
      301 aaagatccag ttcggcacct ttcaacggta acgttgattc aatcagagta atggcattcc
      361 atgaaccgga aagcagcata ataaacgtga actcttctcc cagcatcgcc aggcgactgt
      421 cttcaatatt acagccgcaa ctactgacat gacgggtgat ggtgttcaca attccagggc
      481 gatcggcacc caacgcagtg atcaccagat aatgttgcga tgacagtgtc aaactggtta
      541 ttcctttaag gggtgagttg ttcttaagga aagcataaaa aaaacatgca tacaacaatc
      601 agaacggttc tgtctgcttg cttttaatgc cataccaaac gtaccattga gacacttgtt
      661 tgcacagagg atggcccgaa ttcttgacgg ctagctcagt cctaggtaca gtgctagcgg
      721 atctgctgga acaggtggtg agactcaagg tcatgatgga cgtgaacaaa aaaacgaaaa
      781 ttcgccaccg aaacgagcta aatcacaccc tggctcaact tcctttgccc gcaaagcgag
      841 tgatgtatat ggcgcttgct cccattgata gcaaagaacc tct
//
