LOCUS       sequence-99377-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses T7 RNAP from the arabinose inducible promoter PBAD with a 
            RBS strength of 500 from the Salis RBS calculator. Also includes a 
            constitutive araC ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pTara:500
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     770..789
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 gattatccat cggtggatgg agcgactcgt taatcgcttc catgcgccgc agtaacaatt
       61 gctcaagcag atttatcgcc agcagctccg aatagcgccc ttccccttgc ccggcgttaa
      121 tgatttgccc aaacaggtcg ctgaaatgcg gctggtgcgc ttcatccggg cgaaagaacc
      181 ccgtattggc aaatattgac ggccagttaa gccattcatg ccagtaggcg cgcggacgaa
      241 agtaaaccca ctggtgatac cattcgcgag cctccggatg acgaccgtag tgatgaatct
      301 ctcctggcgg gaacagcaaa atatcacccg gtcggcaaac aaattctcgt ccctgatttt
      361 tcaccacccc ctgaccgcga atggtgagat tgagaatata acctttcatt cccagcggtc
      421 ggtcgataaa aaaatcgaga taaccgttgg cctcaatcgg cgttaaaccc gccaccagat
      481 gggcattaaa cgagtatccc ggcagcaggg gatcattttg cgcttcagcc atacttttca
      541 tactcccgcc attcagagaa gaaaccaatt gtccatattg catcagacat tgccgtcact
      601 gcgtctttta ctggctcttc tcgctaacca aaccggtaac cccgcttatt aaaagcattc
      661 tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg
      721 gcagaaaagt cctaggacat tgattatttg cacggcgtca cactttgcta tgccatagca
      781 tttttatcca taagattagc gg
//
