LOCUS       sequence-99380-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses T7 RNAP from the arabinose inducible promoter PBAD with a 
            RBS strength of 500 from the Salis RBS calculator. Also includes a 
            constitutive araC ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pTara:500
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgcaactctc tactgtttct ccatacccgt ttttttgggc tagcgagctc tagaaagact
       61 tacttaataa cattcttttt ctaatatgaa cacgattaac atcgctaaga acgacttctc
      121 tgacatcgaa ctggctgcta tcccgttcaa cactctggct gaccattacg gtgagcgttt
      181 agctcgcgaa cagttggccc ttgagcatga gtcttacgag atgggtgaag cacgcttccg
      241 caagatgttt gagcgtcaac ttaaagctgg tgaggttgcg gataacgctg ccgccaagcc
      301 tctcatcact accctactcc ctaagatgat tgcacgcatc aacgactggt ttgaggaagt
      361 gaaagctaag cgcggcaagc gcccgacagc cttccagttc ctgcaagaaa tcaagccgga
      421 agccgtagcg tacatcacca ttaagaccac tctggcttgc ctaaccagtg ctgacaatac
      481 aaccgttcag gctgtagcaa gcgcaatcgg tcgggccatt gaggacgagg ctcgcttcgg
      541 tcgtatccgt gaccttgaag ctaagcactt caagaaaaac gttgaggaac aactcaacaa
      601 gcgcgtaggg cacgtctaca agaaagcatt tatgcaagtt gtcgaggctg acatgctctc
      661 taagggtcta ctcggtggcg aggcgtggtc ttcgtggcat aaggaagact ctattcatgt
      721 aggagtacgc tgcatcgaga tgctcattga gtcaaccgga atggttagct tacaccgcca
      781 aaatgctggc gtagtaggtc aagactctga gactatcgaa ctcgcacctg aatacgctga
      841 ggctatcgca acccgtgcag gtgcgctggc tggcatctct ccgatgttcc aaccttgcgt
      901 agttcctcct aag
//
