LOCUS       sequence-99383-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the N-terminal fragment of split T7 RNAP split a position 
            179. Insert is driven by PBAD. Contains a constitutive araC ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pTSara-N
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atctgctcat gtttgacagc
       61 ttatcatcga tgcataatgt gcctgtcaaa tggacgaagc agggattctg caaaccctat
      121 gctactccgt caagccgtca attgtctgat tcgttaccaa ttatgacaac ttgacggcta
      181 catcattcac tttttcttca caaccggcac ggaactcgct cgggctggcc ccggtgcatt
      241 ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga ccgacggtgg
      301 cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc
      361 gccagcttaa gacgctaatc cctaactgct ggcggaaaag atgtgacaga cgcgacggcg
      421 acaagcaaac atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc
      481 tgatgtactg acaagcctcg cgtacccgat tatccatcgg tggatggagc gactcgttaa
      541 tcgcttccat gcgccgcagt aacaattgct caagcagatt tatcgccagc agctccgaat
      601 agcgcccttc cccttgcccg gcgttaatga tttgcccaaa caggtcgctg aaatgcggct
      661 ggtgcgcttc atccgggcga aagaaccccg tattggcaaa tattgacggc cagttaagcc
      721 attcatgcca gtaggcgcgc ggacgaaagt aaacccactg gtgataccat tcgcgagcct
      781 ccggatgacg accgtagtga tgaatctctc ctggcgggaa cagc
//
