LOCUS       sequence-99384-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the N-terminal fragment of split T7 RNAP split a position 
            179. Insert is driven by PBAD. Contains a constitutive araC ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pTSara-N
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      680..707
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 atcgcaactc tctactgttt ctccataccc gtttttttgg gctagcgagc tctagaaaga
       61 cttacttaat aacattcttt ttctaatatg aacacgatta acatcgctaa gaacgacttc
      121 tctgacatcg aactggctgc tatcccgttc aacactctgg ctgaccatta cggtgagcgt
      181 ttagctcgcg aacagttggc ccttgagcat gagtcttacg agatgggtga agcacgcttc
      241 cgcaagatgt ttgagcgtca acttaaagct ggtgaggttg cggataacgc tgccgccaag
      301 cctctcatca ctaccctact ccctaagatg attgcacgca tcaacgactg gtttgaggaa
      361 gtgaaagcta agcgcggcaa gcgcccgaca gccttccagt tcctgcaaga aatcaagccg
      421 gaagccgtag cgtacatcac cattaagacc actctggctt gcctaaccag tgctgacaat
      481 acaaccgttc aggctgtagc aagcgcaatc ggtcgggcca ttgaggacga ggctcgcttc
      541 ggtcgtatcc gtgaccttga agctaagcac ttcaagaaaa acgttgagga acaactcaac
      601 aagcgcgtag ggcacgtcta caagtaatgt ctaacaattc gttcaagccg acggatatcg
      661 agctggcttg gatcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga
      721 gagagaatat aaaaagccag attattaatc cggctttttt attattttgg ctcgtgttga
      781 aatcgcgatg ttgtgtctaa ttttgaagtt aactttgatt ccattctttt gtttgtctgc
      841 cagaacgcag aagc
//
